This record provides an overview of the scope and research output of NESP Marine Biodiversity Hub Project A9 - "Grey Nurse Shark CK-MR Population Estimate – East Coast". For specific data outputs from this project, please see child records associated with this metadata. -------------------- A review of the 2002 National Recovery Plan for Grey Nurse Shark (DEWHA 2009) concluded it was not possible to determine if the east coast population had shown any signs of recovery (DoE 2014); recommending a new recovery plan be developed for this species. A primary objective of the new recovery plan (DoE 2014) is to improve knowledge of GNS population status. This will require a robust estimate of population size and trend – something that has not been provided to date. This project will use genetic SNP data to inform close kin-mark recapture analysis to estimate population size and trend, and provide guidance on future monitoring strategies for the east coast population of grey nurse shark. Planned Outputs • Tools to refine and integrate CK-MR and species demographic data for population assessments of a key threatened species at a national scale (combining knowledge developed under this project combined with similar techniques being applied under NESP to euryhaline sharks and white sharks). • A national estimate of (census) population size and trend for the eastern Australian population of grey nurse shark will be developed to fulfil the highest priority actions of the National Recovery Plan. • Identify national strategies to guide future monitoring of grey nurse shark populations. • The project will provide peer-reviewed additions to the scientific literature that will add to the science-support for the development and implementation of policies to support the ecologically sustainable management of Australia’s marine environment.

This record provides an overview of the NESP Marine and Coastal Hub small-scale study - "A national framework for improving seagrass restoration". For specific data outputs from this project, please see child records associated with this metadata. -------------------- Seagrasses provide resources and ecosystem services critical to the health of coastal ecosystems and human populations. They increase water clarity, stabilise sediments and reduce coastal erosion, sequester carbon, and provide habitat and food to marine animals, including commercially important fish and invertebrates. Across Australia, the loss of >275,000 ha of seagrass meadows and associated ecosystem services—valued at AU$ 5.3 billion—has contributed to the long-term degradation of estuarine and coastal marine ecosystems. Restoration of seagrass is critical for improving the health and function of these ecosystems and sustaining coastal communities and industries that depend on them, yet restoration projects to date typically occur at small scales, driven by local priorities and with variable success. This project addressed this problem by bringing together scientists and key stakeholders to collate knowledge on seagrass ecology and restoration, and generated a framework to scaling-up restoration nationally. A national workshop with experts identified a shortlist of drivers key to restoration success, including sediment dynamics, microbial communities, hydrodynamics, and species interactions. It highlighted the importance of incorporating seagrass genetics and life histories into site and donor material selection to improve long-term resilience. New technologies such as eDNA, automation, and AI were also assessed for their potential to improve monitoring and reduce costs, while standardised methodologies and molecular tools were recommended to track microbial indicators and site suitability. A key insight from the workshop was the central role of sediment processes in feedback loops that determine seagrass health—providing a foundation for more effective, scalable restoration strategies. On-ground case studies were conducted in Western Australia and New South Wales to test the proposed restoration framework in collaboration with Indigenous and community partners: sediment quality assessment and manipulation (Gamay Rangers, UNSW); seed and seedling capture using sediment-filled hessian tubes (Malgana Rangers, UWA); and large-scale seed collection for seed-based restoration through the 'Seeds for Snapper' initiative (OzFish and UWA). These trials demonstrated the effectiveness of community-led restoration and reinforced the potential of seed-based methods for scaling up seagrass recovery. Outputs • Effect of sediment quality and manipulation on seagrass transplant success [field data] • Locations and health of beachcast fragments of Posidonia in Botany Bay [field data] • Effect of engineering hydrodynamics (by use of hessian socks) on seagrass transplant success [field data] • Final project report [written]

This record provides an overview of the NESP Marine and Coastal Hub Research Plan 2023 project "Identifying priority datasets of relevance to the Gippsland declaration area and pathways for their use in guiding decision-making". For specific data outputs from this project, please see child records associated with this metadata. -------------------- Australia has entered a phase of rapid development of offshore renewable energy (ORE) with one declaration area and one notice of proposal to declare an area for ORE infrastructure announced in late 2022 and early 2023 respectively. There is an immediate need to ensure that assessment and regulatory processes can access relevant information on species protected under environmental legislation comprehensively and efficiently, to ensure that decisions are evidence based, gaps in understanding are identified and future research and monitoring is directed to fill those gaps. This project will undertake a rapid exploration of information on a priority subset of species identified by the Department of Climate Change, Energy, Environment and Water (DCCEEW) and the National Offshore Petroleum Safety and Environment Authority (NOPSEMA) identified as critically endangered or endangered under the Environment Protection and Biodiversity Conservation Act 1999 in relation to the Gippsland declaration area. The project aims to 1) identify datasets and information sources relevant to these priority species; 2) identify the level of accessibility of these datasets and information source;, 3) based on the outcomes of 2), evaluate the utility of information identified for assessments required to be undertaken by DCCEEW and NOPSEMA; and 4) identify what activities would need to be undertaken to improve the accessibility and utility of datasets and information sources not currently accessible in useable formats. This project does not intend to duplicate the efforts already being undertaken by NESP project 3.3 in identifying information on marine ecosystems nationally and producing an inventory of recognised best practices for monitoring, mitigation and management of interactions and impacts that can be applied from installation to decommissioning, to be delivered in March 2024, but will fast-track some of the information that can be incorporated into project 3.3. Outputs • Inventory of datasets relevant to the Gippsland OEI declaration area, particularly with respect to priority species identified by DCCEEW and NOPSEMA in association with the Gippsland declaration area [data inventory] • Final project report [written]

This record provides an overview of the scope and research output of NESP Marine Biodiversity Hub Project A12 - "Scoping a seascape approach to managing and recovering Northern Australian threatened and migratory marine species". For specific data outputs from this project, please see child records associated with this metadata. -------------------- Northern Australia is the current focus of substantial economic development, which has the potential to impact biodiversity and cultural values. The Northern Seascape scoping project will assess the status of knowledge of EPBC-listed Threatened and Migratory Marine species, and pressures, Indigenous priorities, habitats, fisheries bycatch, and EPBC referrals in relation to them across the North Marine Bioregion (coast to EEZ edge). The focus will be at the multiple taxa level, including elasmobranchs, shorebirds, turtles and cetaceans. The project will scope research needs and directions for a broad Northern Seascape project (2018–2020), by identifying future research hotspots. Planned Outputs • Maps of Threatened and Migratory Marine species occurrence and habitats, and a gap analysis of research and data needs • Maps of state and trends in pressures and Threatened and Migratory Marine species, and the intersection between them • A report on Indigenous marine research and management priorities for Threatened and Migratory Marine species • Maps and time-series graphs that depict the extent and timing of past changes in coastal habitats that are important for TMM species • Identification of Threatened and Migratory Marine species bycatch and bycatch mitigation research priorities • Identification of EPBC referral spatial and species trends • Data, data visualisation and summaries available online through an appropriate web-based portal and/or existing internal DoEE information products • Project report synthesizing northern Australian Threatened and Migratory Marine species, pressures, Indigenous priorities, coastal habitat change, fisheries bycatch mitigation research priorities, and EPBC referral trends, and the identification of future research hotspots

This record provides an overview of the NESP Marine and Coastal Hub project "Aerial survey of the Southern Right Whale ‘western’ sub-population off southern Australia". For specific data outputs from this project, please see child records associated with this metadata. -------------------- Southern right whales are listed as Endangered under the EPBC Act and are a species of national conservation significance. Monitoring of their recovery is guided by the Southern Right Whale Conservation Management Plan, which aims to improve the population’s conservation status through regular assessment of population size, calving intervals, and spatial trends. This project continued the long-term aerial survey program of southern right whales along the southern Australian coast, spanning from Perth (WA) to Ceduna (SA). Annual surveys have been conducted since 1993, providing a continuous long-term dataset for the 'western' population and supporting national assessments of connectivity with the smaller ‘eastern’ population. The surveys contribute essential data on population trends, calving rates, and movements of individuals. The August 2022 aerial survey ensured an uninterrupted time series in the long-term population trend data. This is particularly important given the species' non-annual breeding cycle (typically every three years). Annual surveys are essential to maintain an acceptable level of precision in estimating population trends and key life history parameters. A total of 526 whales were recorded, including 247 cow–calf pairs, 31 unaccompanied adults and one yearling. Based on long-term models, this equated to a population estimate of approximately 2,675 individuals, with an average annual growth rate of ~5.3%. While this represents a continued population growth, results suggest a possible slowing in the rate of growth over the past 13 years (from 7.5% in 2009). The 2022 survey also recorded the lowest number of unaccompanied animals in the entire time series, extending a five-year trend of low sightings in this group. Continued monitoring of the population is needed to assess whether these changes represent longer-term shifts in population dynamics and calving intervals, and to inform adaptive management for this long-lived, slow-recovering species. Outputs • Estimate of relative abundance and population trend compared to long-term aerial survey sightings [dataset] • Individual whale photo-identification data - 2021-22 season [imagery - published to ARWPIC] • Final technical report detailing overall numbers of southern right whales observed within the survey region, their gender (and life stage where possible) and spatial distribution of individuals [written]

This record provides an overview of the NESP Marine and Coastal Hub bridging study - "A photo-identification study of southern right whales to update aggregation area classification in the southwest of Australia". For specific data outputs from this project, please see child records associated with this metadata. -------------------- The southern right whale (Eubalaena australis) is listed as Endangered under the Environmental Protection and Biodiversity Conservation Act 1999 (EPBC Act) and is subject to conservation listings in five Australian states due to severe population declines caused by historical whaling. The Southern Right Whale Conservation Management Plan 2011–2021 outlines the current status of, and threats to, the southern right whale in Australian waters and prioritises recovery actions during this period. The long-term vision for the recovery of this species in Australian waters is to increase the population size to a level that the conservation status improves, and the species no longer qualifies for listing as threatened under any of the EPBC Act listing criteria. The plan must be periodically updated to reflect new knowledge and prioritise the research needed to monitor population recovery and predict the impacts of threats such as climate change. Aerial surveys of southern right whales have been conducted across the southern Australian coast from Perth (W.A.) to Ceduna (S.A.) since 1993, as part of a long-term program to monitor the recovery, and inform the Conservation Management Plan (2011-2021), for this Endangered species. In Australia’s south-east, there has been little sign of recovery in southern right whale numbers following intense commercial whaling. A working hypothesis assumes separation between the ‘western’ and ‘eastern’ populations, largely due to loss of ‘cultural memory’ of whales migrating to the eastern range breeding areas. Given the relative paucity of animals that visit the southern Australian coast in areas other than south-west Australia, the western population is considered to represent the majority of the ‘Australian’ southern right whale population. The count data from these aerial surveys provide data on population trend and estimates of population size for the ‘western’ population, and hence the majority of the Australian southern right whales. Associated photo-identification data provide life history information (such as calving intervals) and connectivity between the ‘western’ and ‘eastern’ populations and contribute to the national southern right whale photo-id database: the Australasian Right Whale Photo-Identification Catalogue (ARWPIC). The 2020 aerial survey program recorded substantially lower numbers of whales than in the previous 13 years, and the lowest number of non-calving whales since the program started. This project conducted new aerial surveying in August 2021 to provide a relative estimate of annual population size for determining longer term population trends and contribute to determining if 2020 was an anomalous year or an indicator of some longer-term change to recent recovery rates and the female breeding cycle. Outputs • Aerial whale survey data (counts by size class, number, and location) - 2021-22 season [dataset] • Individual whale photo-identification data - 2021-22 season [imagery - published to ARWPIC] • Final Project Report including a short summary of recommendations for policy makers of key findings [written]

This record provides an overview of the NESP Marine and Coastal Hub small-scale study - "Australia’s Coastal Shorebirds: Trends and Prospects". No data outputs were generated by this project. -------------------- Coastal Australia is home to 37 regularly occurring migratory shorebird species, with many protected areas including Ramsar sites designated on the basis of shorebird populations. Many migratory shorebirds are declining rapidly, and hence the focus of conservation efforts at multiple levels of government in Australia and overseas. However, trend data are now nearly 10 years old, meaning the information available to assess where conservation actions are needed most urgently and whether conservation efforts are helping species recover are outdated. To ensure populations have the best chance at recovery and that resources are allocated where they are most likely to have the greatest positive impacts, it is critical to maintain up-to-date information on species trends. This project analysed 30 years of shorebird monitoring data collected by citizen science groups across Australia and curated by BirdLife Australia’s National Shorebird Monitoring Program to update national trend estimates, while also assessing the relative effects of human pressure and conservation efforts on population trends. In particular, it focused on 15 migratory shorebird species whose conservation status was being reassessed by the Australian Department of Climate Change, Energy, the Environment and Water. This project sets the stage for building the next decade of coastal shorebird conservation activity in Australia, coordinated through the national mechanism of the End User: National Migratory Shorebird Conservation Action Plan Steering Committee, with representatives from national and state governments as well as leading shorebird experts. Outputs • Fact Sheet on Australia's coastal shorebird trends and prospects [written] • Final Project Report, including a short summary of recommendations for policy makers of key findings [written]

This record provides an overview of the NESP Marine and Coastal Hub emerging priorities study - "Application of environmental DNA to survey Bathurst Harbour Tasmania for the endangered Maugean skate". For specific data outputs from this project, please see child records associated with this metadata. -------------------- The Maugean skate (Zearaja maugeana) is listed as Endangered under Tasmania’s Threatened Species Protection Act and the Commonwealth Environmental Protection and Biodiversity Conservation Act 1999. Its known population is small (~3,000 individuals, Macquarie Harbour, 2016) and highly restricted, having only been recorded in two isolated estuaries: Bathurst Harbour and Macquarie Harbour in south-western/western Tasmania. This constitutes one of the most limited distributions of any known extant elasmobranch. Although the skate was first discovered in Bathurst Harbour, most knowledge of the species stems from the Macquarie Harbour population. Only four individuals have been reported in Bathurst Harbour, with the last reported sighting in 1992. Environmental conditions in Macquarie Harbour have changed markedly since then due to anthropogenic activities in and around the estuary including historical mining, hydro-electric generation and alteration of natural river flows, and marine fish farming. Previous research has shown clear signs of population stress and evidence of detrimental impacts of degraded environmental conditions on the Maugean Skate in Macquarie Harbour. Understanding the potential population status of the Maugean Skate in Bathurst Harbour will assist in determining conservation actions. This study used Environmental (e) DNA to determine the presence/absence of Maugean skate in Bathurst Harbour, strengthening the evidence base for effective conservation plans and specific recovery actions. Outputs • Maugean skate eDNA sampling data and inferred species distribution (presence/absence) [dataset] • Final Project Report, including a short summary of recommendations for policy makers of key findings [written]

This record provides an overview of the NESP Marine and Coastal Hub Research Plan 2023 project "Eastern Grey Nurse Shark population abundance and trend". For specific data outputs from this project, please see child records associated with this metadata. -------------------- Australia’s eastern grey nurse shark population is listed as Critically Endangered under the Environment Protection and Biodiversity Conservation Act 1999. The Recovery Plan for the Grey Nurse Shark identifies research and actions needed to improve the shark’s population status and ensure its long-term conservation. This project refines the abundance and trend estimate for the eastern Australian population of grey nurse shark and examined the shark’s expansion range into Victorian waters (evidence suggests the shark may now range as far west as Wilsons Promontory). The project builds upon previous research which has estimated the eastern grey nurse shark adult population at ~2,000 individuals. Epigenetic techniques are used estimate age of juveniles (based on patterns of DNA methylation), and stereo video analysis is used to obtain precise length measurements for use against growth curves. Understanding the species' population structure is important to inform the assessment of current conservation arrangements such as spatial closures. Findings will provide a more precise estimate of the abundance of Australia’s eastern grey nurse shark population, and reduce uncertainty surrounding trends in abundance by building a stronger evidence base for decision-making around the species’ recovery and conservation. Outputs • Tissue samples and extracted products from juvenile grey nurse sharks [dataset] • Final project report [written]

This record provides an overview of the NESP Marine and Coastal Hub small-scale study - "Conservation of spotted handfish". For specific data outputs from this project, please see child records associated with this metadata. -------------------- Spotted Handfish (Brachionichthys hirsutus) were once common across the bays and estuaries of South-eastern Tasmania. By 1996, however, populations had declined and the species was listed as Critically Endangered under Australia’s Environment Protection and Biodiversity Conservation Act 1999. The species is relatively short-lived, (5–10 years) and matures at more than two years’ old. This leaves a short window for reproduction, which relies on egg masses laid on seafloor structures such as stalked ascidians. If spawning fails, population declines may occur rapidly. With no planktonic life stage to aid dispersal, and low adult dispersal, outside recruitment to re-establish collapsed populations is unlikely. Analysis of 23 years of Spotted Handfish survey data (1997–2019) supported by previous NESP Marine Biodiversity Hub work (Project A10) helped develop a time-series of survey data, increasing biological understanding and contributing to effective management actions. This project recommenced surveys of multiple local populations, after a two-year gap, to ensure that the potential impacts of development of the Derwent estuary and surrounds handfish populations or their habitats can be detected. Included in the population surveying was identification of suitable locations to plant Artificial Spawning Habitats (ASH) where natural spawning structures have declined. This will continue to support the species' captive breeding program with industry and foster engagement with the indigenous and broader community through participation, talks, outreach, publications, and the National Handfish Recovery Team (NHRT). Outputs • A consolidated database of all available data on spotted handfish imagery, length frequency, and GPS regions to 2022 [time-series database] • Final Project Report, including a short summary of recommendations for policy makers of key findings [written]