This record provides an overview of the NESP Marine and Coastal Hub Research Plan 2024 project "Environmental DNA for measuring offshore marine biodiversity: what can DNA in water collected from the RV Investigator tell us?". For specific data outputs from this project, please see child records associated with this metadata. -------------------- Environmental DNA (eDNA) is genetic material that is found in the environment. In marine ecosystems eDNA is ubiquitous in seawater and is derived from everything from bacteria to invertebrates and fishes. eDNA can be recovered by filtering water samples and then used it to characterise biodiversity. Using environmental DNA (eDNA) found in seawater to get a snapshot of the species present in an area is an emerging technology with diverse applications in marine ecosystem monitoring. This project will collect a large eDNA dataset during the Southeast Australian Marine Ecosystem Survey (SEA-MES). The eDNA samples will be taken from the RV Investigator throughout the water column at offshore sites stretching from Tasmania to NSW, including sites within the South-east Marine Park Network. The voyage includes parallel collection of biodiversity data using a suite of conventional methods (nets, cameras, and acoustics) which will allow for an assessment of how eDNA compliments these approaches. The project will provide a new baseline and unique eDNA-based perspective on the biodiversity of the southeast Australian marine region. It will also allow for evaluation of eDNA sampling methods and guidance for design of effective, scalable, and non-extractive biomonitoring tools for marine ecosystems. Outputs • eDNA sequences with associated collection metadata (x2 voyages x50 sites) [dataset] • Final project report [written]

The National Reef Monitoring Network brings together shallow reef surveys conducted around Australia into a centralised database. The IMOS National Reef Monitoring Network sub-Facility collates, cleans, stores and makes this data rapidly available from contributors including: Reef Life Survey, Parks Australia, Department of Biodiversity, Conservation and Attractions (Western Australia), Department of Environment, Water and Natural Resources (South Australia), Department of Primary Industries (New South Wales), Tasmanian Parks and Wildlife Service and Parks Victoria. The data provided by the National Reef Monitoring Network contributes to establishing and supporting national marine baselines, and assisting with the management of Commonwealth and State marine reserves. The Australian Temperate Reef Network (ATRC) aims to improve biodiversity conservation and the sustainable management of marine resources by coordinating surveys of rocky and coral reefs using scientific methods, with the ultimate goal to improve coastal stewardship. Our activities depend on the skills of marine scientists, experienced and motivated recreational SCUBA divers, partnerships with management agencies and university researchers, and active input from the ATRC partners. ATRC data are freely available to the public for non-profit purposes, so not only managers, but also groups such as local dive clubs or schools may use these data to look at changes over time in their own local reefs. By making data freely available and through public outputs, ATRC aims to raise broader community awareness of the status of Australia?s marine biodiversity and associated conservation issues. This dataset contains data on the cover of macroalage and sessile invertebrates collected in situ at Australian Temperate Reef Collaboration (ATRC) sites. Quadrats are placed at 10 m spacing along each transect line (i.e. 5 per 50 m transect and 20 per contiguous 200 m) by divers skilled in macroalgal identification. The canopy layer, mid-story and substrate are sequentially assessed in each single, 50 -point quadrat.