This dataset was generated to assess the biological impacts of ocean alkalinity enhancement (OAE) in the Southern Ocean. Deck-board incubation experiments were conducted at five sites spanning subantarctic to sea-ice regions during the RV Investigator MISO voyage (January–March 2024). Unfiltered surface seawater was incubated for 5–8 days following additions of three alkalinity sources: sodium hydroxide (NaOH), ground olivine, and steel slag, alongside untreated controls. Measurements collected at the beginning and end of each incubation included carbonate chemistry (total alkalinity and pH), macronutrients (nitrate, phosphate, silicate), chlorophyll-a, biogenic silica, photophysiology (Fv/Fm), and plankton community composition. Phytoplankton and bacterial abundances were quantified using flow cytometry, while genus-level phytoplankton composition was determined by light microscopy. A complementary dark leaching experiment quantified dissolved trace metal release from each OAE material using ICP-MS. The purpose of the study was to distinguish the biological effects of alkalinity change alone from those arising from collateral nutrient and trace-metal release, and to evaluate how different OAE materials influence phytoplankton growth and community structure in iron-limited Southern Ocean waters. The dataset supports assessment of ecological risks and co-benefits associated with ocean alkalinity enhancement as a carbon dioxide removal strategy.

Southern Ocean phytoplankton growth is limited by low iron (Fe) supply and irradiance, impacting the strength of the biological carbon pump. Unfavourable upper ocean conditions such as low nutrient concentrations can lead to the formation of deep chlorophyll or biomass maxima (DCM/DBM). While common in the Southern Ocean, these features remain under-studied due to their subsurface location. To increase our understanding of their occurrence, we studied the responses of phytoplankton communities from a Southern Ocean DCM to increasing light, Fe, and manganese (Mn) levels. The DCM communities were light- and Fe-limited, but light limitation did not increase phytoplankton Fe requirements. The greatest physiological responses were observed under combined Fe/light additions, which stimulated macronutrient drawdown, biomass production and the growth of large diatoms. Combined Mn/light additions induced subtle changes in Fe uptake rates and community composition, suggesting species-specific Mn requirements. These results provide valuable information on Southern Ocean DCM phytoplankton physiology.

Conducted under the Coast and Clean Seas program of the Natural Heritage Trust fund, this study provides a summary and assessment of water quality parameters, as indicators of estuarine health, in 22 selected Tasmanian estuaries. The estuaries are: Duck Bay, East Inlet, Black River, Don River, Mersey River, Port Sorell, Boobyalla Inlet, Little Musselroe River, Ansons Bay, Grants Lagoon, Douglas River, Great Swanport, Meredith River, Little Swanport, Earlham Lagoon, Browns River, Cloudy Bay Lagoon, Catamaran River, Cockle Creek, Pieman River, Nelson Bay River and Arthur River (for location, see Fig. 1 in the attached report). Information is summarised on both a State wide and individual estuary basis.

A 12-month program was developed and implemented in order to obtain baseline information on water quality (salinity, water temperature, dissolved oxygen, turbidity, pH, dissolved nutrients, silica), ecological condition as shown by Chlorophyll a, benthic macroinvertebrates, pathogens, and habitat extent determined from habitat mapping. Five key estuaries and coastal waters were assessed in the Southern NRM Region of Tasmania. This data includes sampling from Pitt Water / Orielton Lagoon, North West Bay, Port Cygnet, Little Swanport, Moulting Lagoon / Great Swanport.

This study considered a range of water-column and sediment (benthos) based variables commonly used to monitor estuaries,utilising estuaries on the North-West Coast of Tasmania (Duck, Montagu, Detention, and Black River). These included: salinity, dissolved oxygen, turbidity, nutrient and chlorophyll a levels for the water-column; and sediment redox, organic carbon content, chlorophyll a and macroinvertebrate community structure amongst the benthos. In addition to comparing reference with impacted estuaries, comparisons were also made across seasons, commensurate with seasonal changes in freshwater river input, and between regions within estuaries (upper and lower reaches) - previously identified in Hirst et al. (2005). This design enabled us to examine whether the detection of impacts (i.e. differences between reference and impacted systems) was contingent on the time and location of sampling or independent of these factors. This data includes sampling from Duck River, Montagu River, Detention River, and Black River.

Biologically relevant macronutrients, nitrate + nitrite, silicate, phosphate and ammonia, were measured at all sites throughout the study. Nitrate + nitrite values (NOx) at the surface showed clear seasonal trends, peaking over winter and drawing down to near zero in summer and autumn. Phosphate concentrations also reached a peak in winter, which was associated with Southern Ocean influence. Median ammonium concentrations at all sites were generally <0.5 μM, with no clear peaks in any season or month. Overall, the lowest values were measured in August and other months showed reasonable spread around the median. Median silicate concentrations were consistently highest at sites 1 and 9, followed by site 5. Water from the River Derwent flows through site 1, then tracks east towards site 9 then site 5. Seasonally, silicate was generally highest in winter when the River Derwent outflow is also greatest.