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This data presents the results of seabed mapping and habitat classification surveys completed in Darwin Harbour during 2011 and 2013 as part of the Northern Territory Government's marine habitat mapping program. This research is a collaboration between Geoscience Australia (GA), the Australian Institute of Marine Science (AIMS), the Department of Land Resource Management (DLRM) and the Darwin Port Corporation. Key objectives are to: - Produce detailed maps of the bathymetry and derived parameters such as slope and rugosity, - Classify the seabed into areas of hard and soft substrate, and, - Produce seabed habitat maps (or seascapes). Key outcomes from the surveys include: 1. Improved understanding of the seabed of Darwin Harbour. The main seabed geomorphic features identified in Darwin Harbour include banks, ridges, plains and scarps, and a deep central channel that divides into smaller and shallower channels. Acoustically hard substrates are found mostly on banks and are associated with rocky reef and sponge gardens, and are often overlain by a thin veneer of sandy sediment. In contrast, plains and channels are characterised by acoustically soft substrates and are associated with fine sediments (mud and sand). 2. Classification of physical seabed properties to produce a Seascape Map for Darwin Harbour. Six seascape classes (potential habitats) were derived using an Iterative Self Organising (ISO) unsupervised classification scheme. These six classes are related to statistically unique combinations of seabed substrate, relief, bedform and presence of sediment veneer (quite often inferred from presence of epibenthic biota).