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  • Adult and sub-adult Red handfish (Thymichthys politus) and Spotted handfish (Brachionichthys hirsutus) preserved specimens and underwater images were used for analysing morphometrics (comprising of specimens from the CSIRO Australian National Fish Collection and underwater images). Individuals were measured for the morphological traits using electronic callipers (±0.1 mm) for preserved specimens and using Image J software for digital records. Note digital image size calibration occurred using a ruler in images or from size taken in situ. The purpose was to investigate whether external morphometrics could be used to determine sex in handfishes.