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    Sustainable finfish aquaculture is dependent on a benthic environment that can assimilate and process farm particulate wastes. In Macquarie Harbour, bottom and mid water Dissolved Oxygen levels have reached very low levels, which is associated with an increase in the presence of bacterial mats and a significant decline in the abundance and diversity of benthic fauna. Dissolved Oxygen levels are a major determinant of the response of benthic communities in the harbour over timescales of months to years. This dataset consist of dissolved oxygen (DO) and temperature data collected using HOBO Dissolved Oxygen loggers (U26-001) deployed at two locations in Macquarie Harbour, Tasmania under FRDC project 2016-067.