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This dataset consists of data collected by the real-time dissolved oxygen (DO) monitoring system in Macquarie Harbour, Tasmania, originally established under the Sense-T program, continued under FRDC project 2016-067 and deployed and maintained by IMAS. The system consists of 3 strings of acoustically telemetered, optical fluorescence DO, temperature and depth sensors (VEMCO, Bedford, Canada) which measure DO in % saturation, temperature in °C and depth in meters. The dataset spanning the time period from the 1st of January 2017 to the 19th of April 2017 consists of data collected using the first generation of sensors as described above. Subsequent quarterly updates from the 3rd of June 2017 onwards consist of data collected using the new generation of tags which contain a tilt instead of a depth sensor.