To investigate how the unavoidable physical and chemical perturbations associated with Ocean Alkalinity Enhancement (OAE) could influence marine plankton communities and how potential side-effects compare to impacts of climate change, we conducted 19 ship-based experiments in the Equatorial Pacific, examining three prevalent OAE source (NaOH, olivine, and steel slag) and their impacts on natural phytoplankton populations. Our experiments simulated realistic and moderate alkalinity enhancements between 29-16 μmol kg-1. The monitored parameters included total chlorophyll-a concentrations, macro nutrients, trace elements, total alkalinity, Fv/Fm, pH,and flowcytometry.

To understand the environmental impacts of added alkaline minerals on plankton communities, we enclosed natural coastal plankton communities using 53L microcosms and exposed these communities to ground factory slag (2g/53L) and olivine (100g/53L). The microcosms of seawater were kept at 13.5 °C with circulations. The biochemical changes and responses in microcosms were monitored and measured for 21 days. The measured parameters are pH, total alkalinity, temperature, macro-nutrients concentrations, total chlorophyll-a, flow cytometry data, POC/PON, BSi, Rapid Light Curves, zooplankton abundance, the dissolved trace metal concentrations, and the particulate trace metal concentrations.