Date (Publication)

2023-03-15T00:00:00

Credit

This research has been supported by the Australian Research Council through a Future Fellowship awarded to Lennart Thomas Bach (project FT200100846). It's also supported by Australian Antarctic Program Partnership (AAPP), Institute for Marine and Antarctic Studies, University of Tasmania, Hobart, Tasmania, Australia.

Status

Completed

Topic category

• Biota

Maintenance and update frequency

Not planned

Global Change Master Directory Earth Science Keywords, Version 8.5

• EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS
• EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | AQUATIC ECOSYSTEMS | PLANKTON
• EARTH SCIENCE | OCEANS | OCEAN CHEMISTRY | ALKALINITY
• EARTH SCIENCE | OCEANS | OCEAN CHEMISTRY | CARBON DIOXIDE
• EARTH SCIENCE | TERRESTRIAL HYDROSPHERE | WATER QUALITY/WATER CHEMISTRY | CONTAMINANTS | TRACE METALS

AODN Discovery Parameter Vocabulary

• pH (total scale) of the water body
• Temperature of the water body
• voltage
• Mesocosm No.
• Day
• Concentration of biogenic silicate in the seawater
• Total alkalinity per unit mass of the water body
• Concentration of chlorophyll-a per unit volume of the water body
• Concentration of suspended particulate nitrogen
• Concentration of suspended particulate carbon
• Concentration of nitrate and nitrite {NO3 and NO2} per unit mass of the water body
• Concentration of phosphate {PO4} per unit mass of the water body
• Concentration of silicate {SiO4} per unit mass of the water body
• Biotic taxonomic identification
• Abundance of biota
• Mean forward scatter signal values
• Concentration of dissolved metal in seawater
• Name of the dissolved metal in seawater
• The initial slope of rapid light curve
• The maximum electron transport rate
• The minimum saturation irradiance
• The maximum quantum yield of photosynthesis II

AODN Geographic Extents Vocabulary

• Coastal Cities / Towns (Australia) | Coastal Cities / Towns (Australia) | Hobart, TAS

Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research

• Biological Oceanography
• Marine and Estuarine Ecology (incl. Marine Ichthyology)

Language

English

Character encoding

UTF8

Content type

Physical measurement

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UUUU

Name

Dimensionless

Name

T

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UPKA

Name

Degrees Kelvin

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UVLT

Name

Volts

Name

mesocosm

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UUUU

Name

Dimensionless

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UUUU

Name

Dimensionless

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UPOX

Name

Micromoles per litre

Name

TA

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/KGUM

Name

Micromoles per kilogram

Name

Chla

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UGPL

Name

Micrograms per litre

Name

N

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UPOX

Name

Micromoles per litre

Name

C

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UPOX

Name

Micromoles per litre

Name

N

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UPOX

Name

Micromoles per litre

Name

P

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UPOX

Name

Micromoles per litre

Name

Si

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UPOX

Name

Micromoles per litre

Name

Species

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UUUU

Name

Dimensionless

Name

Number

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UUUU

Name

Dimensionless

Name

Mean FSC-A

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UUUU

Name

Dimensionless

Name

Cell count

Name

cell count per microliter

Name

Concentration3

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UPOX

Name

Micromoles per litre

Name

Isotope

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UUUU

Name

Dimensionless

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UUUU

Name

Dimensionless

Name

ETRmax

Name

micromole electrons per square meters per second

Name

Ek

Name

micromole quanta per square meters per second

Name

Fv/Fm

Identifier

http://vocab.nerc.ac.uk/collection/P06/current/UUUU

Name

Dimensionless

Distribution format

• Microsoft Excel, CSV

OnLine resource

DATA ACCESS - download data package as zip file

Statement

1. The seawater’s chemical characteristics including salinity, pH, total alkalinity (TA), and macro-nutrients were monitored throughout the experiment. All seawater sampling processes were done 15cm deep from the surface using a pump and acid-washed silicate tubes. pH and temperature were measured every morning using a pH meter (914 pH/Conductometer Metrohm). TA was measured using Metrohm 862 Compact Titrosampler coupled with an Aquatrode Plus with PT1000 temperature sensor following the SOP3b open-cell titration protocol described in Dickson et al. (2017). 2. Dissolved macro-nutrients were measured using spectrophotometric methods developed by Hansen and Koroleff (1999). 3. Dissolved trace metal:60ml of seawater was collected using an acid-washed 60ml syringe, and the seawater was filtered by 0.2µm pore size polycarbonate syringe filters. Phytoplankton was sampled before the treatment and at the end of the experiment for the analysis of trace metal concentration inside phytoplankton cells. Then the phytopalnkton were digested using strong acid to extract the trace metals. All trace metal concentrations were measured using ICP-MS. 4. The particulate organic carbon and particulate organic nitrogen (POC and PON) and biogenic silica (BSi) were analysed every 4 days by filtering 100ml/filter seawater collected seawater from each microcosm. 5. An all-plastic of zooplankton net (20mm high and 15mm width) with a 210µm pole size mesh was used to collect zooplankton from microcosms before addition (day 2), in the middle (day 13) and at the end of the experiment (day 23), and zooplankton was observed under a microscope to distinguish their taxon groups. 6. Chlorophyll a was sampled every 2 days by filtering the seawater through glass fibre filters (GF/F, pore size = 0.7 µm, diameter =25mm). Each filter was immersed in 10ml 100% methanol for 18-20h to extract chlorophyll from phytoplankton and these samples were analysed on a Turner fluorometer (10-AU) following the method described by Evans, et al. (1987). 7. Flow cytometry samples were subsampled using pipettes from the pumped seawater samples. A mixture of formaldehyde-hexamine (18%:10% v/w) was added to preserve phytoplankton samples at 2.8%. All bacteria samples were fixed using glutaraldehyde (Electron-microscope grade, 25%) at 2.0%. Before the measurement, samples were thawed at 37℃. Bacteria samples were stained with SYBR green I (diluted in dimethylsulfoxide) at a final ratio of 1:10000 (SYBR Green I : sample) prior to analysis. The Cytek Aurora (Cytek Biosciences) was used to measure fluorescence signal intensity from different channels and forward scatter (FSC) information of phytoplankton cells. 8. Phytoplankton photosynthesis performance was estimated from the rapid light curves measured with Fast Repetition Rate (FRR) fluorometry every 2 days (Schallenberg et al. 2020). Samples were kept in dark for 20 minutes before the measurements. Live phytoplankton samples were added to the FRR fluorometry cuvette, which is temperature-controlled at 13.5°C.

Hierarchy level

Dataset

Resource scope

Dataset

Name

IMAS Dataset level record

Title

ISO 19115-3:2018