Microcosm experiment on Ocean Alkalinity Enhancement 2023

To understand the environmental impacts of added alkaline minerals on plankton communities, we enclosed natural coastal plankton communities using 53L microcosms and exposed these communities to ground factory slag (2g/53L) and olivine (100g/53L). The microcosms of seawater were kept at 13.5 °C with circulations. The biochemical changes and responses in microcosms were monitored and measured for 21 days. The measured parameters are pH, total alkalinity, temperature, macro-nutrients concentrations, total chlorophyll-a, flow cytometry data, POC/PON, BSi, Rapid Light Curves, zooplankton abundance, the dissolved trace metal concentrations, and the particulate trace metal concentrations.

Simple

Identification info

Date (Publication)
2023-03-15T00:00:00

Resource provider

Institute for Marine and Antarctic Studies (IMAS)
Private Bag 129
Hobart
Tasmania
7001
Australia

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Guo, Jiaying
Private Bag 129
Hobart
TAS
7001
Australia
ORCID ID >

Collaborator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Bach, Lennart
Private Bag 129
Hobart
TAS
7001
Australia
ORCID ID >

Credit
This research has been supported by the Australian Research Council through a Future Fellowship awarded to Lennart Thomas Bach (project FT200100846). It's also supported by Australian Antarctic Program Partnership (AAPP), Institute for Marine and Antarctic Studies, University of Tasmania, Hobart, Tasmania, Australia.
Status
Completed

Resource provider

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Guo, Jiaying
Hobart
TAS
7001
Australia
ORCID ID >

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Bach, Lennart
Hobart
TAS
7001
Australia
ORCID ID >

Topic category
  • Biota

Extent

Temporal extent

Time period
2022-05-23 2022-11-01
Maintenance and update frequency
Not planned
Global Change Master Directory Earth Science Keywords, Version 8.5
AODN Discovery Parameter Vocabulary
AODN Geographic Extents Vocabulary
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research

Resource constraints

Use limitation
Data, products and services from IMAS are provided "as is" without any warranty as to fitness for a particular purpose.

Resource constraints

Other constraints
This dataset is the intellectual property of the University of Tasmania (UTAS) through the Institute for Marine and Antarctic Studies (IMAS).

Resource constraints

Linkage
https://licensebuttons.net/l/by/4.0/88x31.png

License Graphic

Title
Creative Commons Attribution 4.0 International License
Alternate title
CC-BY-NC
Edition
4.0


>

Website
https://creativecommons.org/licenses/by/4.0/

License Text

Other constraints
Cite data as: Guo, J., & Bach, L. (2023). Microcosm experiment on Ocean Alkalinity Enhancement 2023 [Data set]. Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS). https://doi.org/10.25959/X6FH-9K15
Language
English
Character encoding
UTF8

Content Information

Content type
Physical measurement

Identifier

Code
pH
Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UUUU
Name
Dimensionless

Identifier

Code
Temperature of the water body
Name
T

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UPKA
Name
Degrees Kelvin

Identifier

Code
voltage
Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UVLT
Name
Volts

Identifier

Code
Mesocosm No.
Name
mesocosm

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UUUU
Name
Dimensionless

Identifier

Code
Day
Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UUUU
Name
Dimensionless

Identifier

Code
Concentration of biogenic silicate in the seawater
Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UPOX
Name
Micromoles per litre

Identifier

Code
Total alkalinity per unit mass of the water body
Name
TA

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/KGUM
Name
Micromoles per kilogram

Identifier

Code
Concentration of chlorophyll-a per unit volume of the water body
Name
Chla

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UGPL
Name
Micrograms per litre

Identifier

Code
Concentration of suspended particulate nitrogen
Name
N

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UPOX
Name
Micromoles per litre

Identifier

Code
Concentration of suspended particulate carbon
Name
C

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UPOX
Name
Micromoles per litre

Identifier

Code
Concentration of nitrate and nitrite {NO3 and NO2} per unit mass of the water body
Name
N

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UPOX
Name
Micromoles per litre

Identifier

Code
Concentration of phosphate {PO4} per unit mass of the water body
Name
P

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UPOX
Name
Micromoles per litre

Identifier

Code
Concentration of silicate {SiO4} per unit mass of the water body
Name
Si

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UPOX
Name
Micromoles per litre

Identifier

Code
Name of the species taxon
Name
Species

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UUUU
Name
Dimensionless

Identifier

Code
Abundance of biota
Name
Number

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UUUU
Name
Dimensionless

Identifier

Code
Mean forward scatter signal values
Name
Mean FSC-A

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UUUU
Name
Dimensionless

Identifier

Code
Abundance of biota
Name
Cell count

Name
cell count per microliter

Identifier

Code
Concentration of dissolved metal in seawater
Name
Concentration3

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UPOX
Name
Micromoles per litre

Identifier

Code
Name of the dissolved metal in seawater
Name
Isotope

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UUUU
Name
Dimensionless

Identifier

Code
The initial slope of rapid light curve
Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UUUU
Name
Dimensionless

Identifier

Code
The maximum electron transport rate
Name
ETRmax

Name
micromole electrons per square meters per second

Identifier

Code
The minimum saturation irradiance
Name
Ek

Name
micromole quanta per square meters per second

Identifier

Code
The maximum quantum yield of photosynthesis II
Name
Fv/Fm

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UUUU
Name
Dimensionless

Distribution Information

Distribution format
  • Microsoft Excel, CSV

OnLine resource
DATA ACCESS - download data package as zip file

Resource lineage

Statement
1. The seawater’s chemical characteristics including salinity, pH, total alkalinity (TA), and macro-nutrients were monitored throughout the experiment. All seawater sampling processes were done 15cm deep from the surface using a pump and acid-washed silicate tubes. pH and temperature were measured every morning using a pH meter (914 pH/Conductometer Metrohm). TA was measured using Metrohm 862 Compact Titrosampler coupled with an Aquatrode Plus with PT1000 temperature sensor following the SOP3b open-cell titration protocol described in Dickson et al. (2017). 2. Dissolved macro-nutrients were measured using spectrophotometric methods developed by Hansen and Koroleff (1999). 3. Dissolved trace metal:60ml of seawater was collected using an acid-washed 60ml syringe, and the seawater was filtered by 0.2µm pore size polycarbonate syringe filters. Phytoplankton was sampled before the treatment and at the end of the experiment for the analysis of trace metal concentration inside phytoplankton cells. Then the phytopalnkton were digested using strong acid to extract the trace metals. All trace metal concentrations were measured using ICP-MS. 4. The particulate organic carbon and particulate organic nitrogen (POC and PON) and biogenic silica (BSi) were analysed every 4 days by filtering 100ml/filter seawater collected seawater from each microcosm. 5. An all-plastic of zooplankton net (20mm high and 15mm width) with a 210µm pole size mesh was used to collect zooplankton from microcosms before addition (day 2), in the middle (day 13) and at the end of the experiment (day 23), and zooplankton was observed under a microscope to distinguish their taxon groups. 6. Chlorophyll a was sampled every 2 days by filtering the seawater through glass fibre filters (GF/F, pore size = 0.7 µm, diameter =25mm). Each filter was immersed in 10ml 100% methanol for 18-20h to extract chlorophyll from phytoplankton and these samples were analysed on a Turner fluorometer (10-AU) following the method described by Evans, et al. (1987). 7. Flow cytometry samples were subsampled using pipettes from the pumped seawater samples. A mixture of formaldehyde-hexamine (18%:10% v/w) was added to preserve phytoplankton samples at 2.8%. All bacteria samples were fixed using glutaraldehyde (Electron-microscope grade, 25%) at 2.0%. Before the measurement, samples were thawed at 37℃. Bacteria samples were stained with SYBR green I (diluted in dimethylsulfoxide) at a final ratio of 1:10000 (SYBR Green I : sample) prior to analysis. The Cytek Aurora (Cytek Biosciences) was used to measure fluorescence signal intensity from different channels and forward scatter (FSC) information of phytoplankton cells. 8. Phytoplankton photosynthesis performance was estimated from the rapid light curves measured with Fast Repetition Rate (FRR) fluorometry every 2 days (Schallenberg et al. 2020). Samples were kept in dark for 20 minutes before the measurements. Live phytoplankton samples were added to the FRR fluorometry cuvette, which is temperature-controlled at 13.5°C.
Hierarchy level
Dataset

Metadata

Metadata identifier
urn:uuid/743b1215-66b4-4803-9e4e-6e8c503fe8a8

Language
English
Character encoding
UTF8

Distributor

IMAS Data Manager - Data Officer
Institute for Marine and Antarctic Studies
Private Bag 129
Hobart
Tasmania
7001
Australia
Institute for Marine and Antarctic Studies (IMAS) website >

Type of resource

Resource scope
Dataset
Name
IMAS Dataset level record
Metadata linkage
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/743b1215-66b4-4803-9e4e-6e8c503fe8a8

Point of truth URL of this metadata record

Date info (Creation)
2023-03-13T00:00:00
Date info (Revision)
2023-10-30T11:19:03

Metadata standard

Title
ISO 19115-3:2018
 
 

Overviews

Spatial extent

Keywords

AODN Discovery Parameter Vocabulary
Abundance of biota Biotic taxonomic identification Concentration of biogenic silicate in the seawater Concentration of chlorophyll-a per unit volume of the water body Concentration of dissolved metal in seawater Concentration of nitrate and nitrite {NO3 and NO2} per unit mass of the water body Concentration of phosphate {PO4} per unit mass of the water body Concentration of silicate {SiO4} per unit mass of the water body Concentration of suspended particulate carbon Concentration of suspended particulate nitrogen Day Mean forward scatter signal values Mesocosm No. Name of the dissolved metal in seawater Temperature of the water body The initial slope of rapid light curve The maximum electron transport rate The maximum quantum yield of photosynthesis II The minimum saturation irradiance Total alkalinity per unit mass of the water body pH (total scale) of the water body voltage
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
Biological Oceanography Marine and Estuarine Ecology (incl. Marine Ichthyology)
Global Change Master Directory Earth Science Keywords, Version 8.5
EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | AQUATIC ECOSYSTEMS | PLANKTON EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS EARTH SCIENCE | OCEANS | OCEAN CHEMISTRY | ALKALINITY EARTH SCIENCE | OCEANS | OCEAN CHEMISTRY | CARBON DIOXIDE EARTH SCIENCE | TERRESTRIAL HYDROSPHERE | WATER QUALITY/WATER CHEMISTRY | CONTAMINANTS | TRACE METALS

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