Microcosm experiment on Ocean Alkalinity Enhancement 2023
To understand the environmental impacts of added alkaline minerals on plankton communities, we enclosed natural coastal plankton communities using 53L microcosms and exposed these communities to ground factory slag (2g/53L) and olivine (100g/53L). The microcosms of seawater were kept at 13.5 °C with circulations. The biochemical changes and responses in microcosms were monitored and measured for 21 days. The measured parameters are pH, total alkalinity, temperature, macro-nutrients concentrations, total chlorophyll-a, flow cytometry data, POC/PON, BSi, Rapid Light Curves, zooplankton abundance, the dissolved trace metal concentrations, and the particulate trace metal concentrations.
Simple
Identification info
- Date (Publication)
- 2023-03-15T00:00:00
Resource provider
Principal investigator
Collaborator
- Credit
- This research has been supported by the Australian Research Council through a Future Fellowship awarded to Lennart Thomas Bach (project FT200100846). It's also supported by Australian Antarctic Program Partnership (AAPP), Institute for Marine and Antarctic Studies, University of Tasmania, Hobart, Tasmania, Australia.
- Status
- Completed
Resource provider
Point of contact
- Topic category
-
- Biota
Temporal extent
- Time period
- 2022-05-23 2022-11-01
- Maintenance and update frequency
- Not planned
- Global Change Master Directory Earth Science Keywords, Version 8.5
-
- EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS
- EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | AQUATIC ECOSYSTEMS | PLANKTON
- EARTH SCIENCE | OCEANS | OCEAN CHEMISTRY | ALKALINITY
- EARTH SCIENCE | OCEANS | OCEAN CHEMISTRY | CARBON DIOXIDE
- EARTH SCIENCE | TERRESTRIAL HYDROSPHERE | WATER QUALITY/WATER CHEMISTRY | CONTAMINANTS | TRACE METALS
- AODN Discovery Parameter Vocabulary
-
- pH (total scale) of the water body
- Temperature of the water body
- voltage
- Mesocosm No.
- Day
- Concentration of biogenic silicate in the seawater
- Total alkalinity per unit mass of the water body
- Concentration of chlorophyll-a per unit volume of the water body
- Concentration of suspended particulate nitrogen
- Concentration of suspended particulate carbon
- Concentration of nitrate and nitrite {NO3 and NO2} per unit mass of the water body
- Concentration of phosphate {PO4} per unit mass of the water body
- Concentration of silicate {SiO4} per unit mass of the water body
- Biotic taxonomic identification
- Abundance of biota
- Mean forward scatter signal values
- Concentration of dissolved metal in seawater
- Name of the dissolved metal in seawater
- The initial slope of rapid light curve
- The maximum electron transport rate
- The minimum saturation irradiance
- The maximum quantum yield of photosynthesis II
Resource constraints
- Use limitation
- Data, products and services from IMAS are provided "as is" without any warranty as to fitness for a particular purpose.
Resource constraints
- Other constraints
- This dataset is the intellectual property of the University of Tasmania (UTAS) through the Institute for Marine and Antarctic Studies (IMAS).
Resource constraints
- Linkage
-
https://licensebuttons.net/l/by/4.0/88x31.png
License Graphic
- Title
- Creative Commons Attribution 4.0 International License
- Alternate title
- CC-BY-NC
- Edition
- 4.0
- Website
-
https://creativecommons.org/licenses/by/4.0/
License Text
- Other constraints
- Cite data as: Guo, J., & Bach, L. (2023). Microcosm experiment on Ocean Alkalinity Enhancement 2023 [Data set]. Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS). https://doi.org/10.25959/X6FH-9K15
- Language
- English
- Character encoding
- UTF8
Content Information
- Content type
- Physical measurement
Identifier
- Code
- pH
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UUUU
- Name
- Dimensionless
Identifier
- Name
- T
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPKA
- Name
- Degrees Kelvin
Identifier
- Code
- voltage
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UVLT
- Name
- Volts
Identifier
- Code
- Mesocosm No.
- Name
- mesocosm
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UUUU
- Name
- Dimensionless
Identifier
- Code
- Day
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UUUU
- Name
- Dimensionless
Identifier
- Code
- Concentration of biogenic silicate in the seawater
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPOX
- Name
- Micromoles per litre
Identifier
- Name
- TA
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/KGUM
- Name
- Micromoles per kilogram
Identifier
- Name
- Chla
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UGPL
- Name
- Micrograms per litre
Identifier
- Code
- Concentration of suspended particulate nitrogen
- Name
- N
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPOX
- Name
- Micromoles per litre
Identifier
- Code
- Concentration of suspended particulate carbon
- Name
- C
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPOX
- Name
- Micromoles per litre
- Name
- N
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPOX
- Name
- Micromoles per litre
Identifier
- Name
- P
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPOX
- Name
- Micromoles per litre
Identifier
- Name
- Si
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPOX
- Name
- Micromoles per litre
Identifier
- Code
- Name of the species taxon
- Name
- Species
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UUUU
- Name
- Dimensionless
Identifier
- Code
- Abundance of biota
- Name
- Number
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UUUU
- Name
- Dimensionless
Identifier
- Code
- Mean forward scatter signal values
- Name
- Mean FSC-A
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UUUU
- Name
- Dimensionless
Identifier
- Code
- Abundance of biota
- Name
- Cell count
- Name
- cell count per microliter
Identifier
- Code
- Concentration of dissolved metal in seawater
- Name
- Concentration3
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPOX
- Name
- Micromoles per litre
Identifier
- Code
- Name of the dissolved metal in seawater
- Name
- Isotope
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UUUU
- Name
- Dimensionless
Identifier
- Code
- The initial slope of rapid light curve
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UUUU
- Name
- Dimensionless
Identifier
- Code
- The maximum electron transport rate
- Name
- ETRmax
- Name
- micromole electrons per square meters per second
Identifier
- Code
- The minimum saturation irradiance
- Name
- Ek
- Name
- micromole quanta per square meters per second
Identifier
- Code
- The maximum quantum yield of photosynthesis II
- Name
- Fv/Fm
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UUUU
- Name
- Dimensionless
Distribution Information
- Distribution format
-
- Microsoft Excel, CSV
- OnLine resource
- DATA ACCESS - download data package as zip file
Resource lineage
- Statement
- 1. The seawater’s chemical characteristics including salinity, pH, total alkalinity (TA), and macro-nutrients were monitored throughout the experiment. All seawater sampling processes were done 15cm deep from the surface using a pump and acid-washed silicate tubes. pH and temperature were measured every morning using a pH meter (914 pH/Conductometer Metrohm). TA was measured using Metrohm 862 Compact Titrosampler coupled with an Aquatrode Plus with PT1000 temperature sensor following the SOP3b open-cell titration protocol described in Dickson et al. (2017). 2. Dissolved macro-nutrients were measured using spectrophotometric methods developed by Hansen and Koroleff (1999). 3. Dissolved trace metal:60ml of seawater was collected using an acid-washed 60ml syringe, and the seawater was filtered by 0.2µm pore size polycarbonate syringe filters. Phytoplankton was sampled before the treatment and at the end of the experiment for the analysis of trace metal concentration inside phytoplankton cells. Then the phytopalnkton were digested using strong acid to extract the trace metals. All trace metal concentrations were measured using ICP-MS. 4. The particulate organic carbon and particulate organic nitrogen (POC and PON) and biogenic silica (BSi) were analysed every 4 days by filtering 100ml/filter seawater collected seawater from each microcosm. 5. An all-plastic of zooplankton net (20mm high and 15mm width) with a 210µm pole size mesh was used to collect zooplankton from microcosms before addition (day 2), in the middle (day 13) and at the end of the experiment (day 23), and zooplankton was observed under a microscope to distinguish their taxon groups. 6. Chlorophyll a was sampled every 2 days by filtering the seawater through glass fibre filters (GF/F, pore size = 0.7 µm, diameter =25mm). Each filter was immersed in 10ml 100% methanol for 18-20h to extract chlorophyll from phytoplankton and these samples were analysed on a Turner fluorometer (10-AU) following the method described by Evans, et al. (1987). 7. Flow cytometry samples were subsampled using pipettes from the pumped seawater samples. A mixture of formaldehyde-hexamine (18%:10% v/w) was added to preserve phytoplankton samples at 2.8%. All bacteria samples were fixed using glutaraldehyde (Electron-microscope grade, 25%) at 2.0%. Before the measurement, samples were thawed at 37℃. Bacteria samples were stained with SYBR green I (diluted in dimethylsulfoxide) at a final ratio of 1:10000 (SYBR Green I : sample) prior to analysis. The Cytek Aurora (Cytek Biosciences) was used to measure fluorescence signal intensity from different channels and forward scatter (FSC) information of phytoplankton cells. 8. Phytoplankton photosynthesis performance was estimated from the rapid light curves measured with Fast Repetition Rate (FRR) fluorometry every 2 days (Schallenberg et al. 2020). Samples were kept in dark for 20 minutes before the measurements. Live phytoplankton samples were added to the FRR fluorometry cuvette, which is temperature-controlled at 13.5°C.
- Hierarchy level
- Dataset
Metadata
- Metadata identifier
- urn:uuid/743b1215-66b4-4803-9e4e-6e8c503fe8a8
- Language
- English
- Character encoding
- UTF8
Distributor
Type of resource
- Resource scope
- Dataset
- Name
- IMAS Dataset level record
- Metadata linkage
-
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/743b1215-66b4-4803-9e4e-6e8c503fe8a8
Point of truth URL of this metadata record
- Date info (Creation)
- 2023-03-13T00:00:00
- Date info (Revision)
- 2023-10-30T11:19:03
Metadata standard
- Title
- ISO 19115-3:2018