Ecophysiology of three habitat-forming seaweeds across a depth and latitudinal gradient in southeastern Australia

Seasonal patterns in the in situ ecophysiology of the common habitat-forming seaweeds Ecklonia radiata, Phyllospora comosa, and Macrocystis pyrifera were investigated at different latitudes and depths in southeastern Australia. We used multiple performance indicators (photosynthetic characteristics, pigment content, chemical composition, stable isotopes, nucleic acids) to assess the ecophysiology of seaweeds near the northern and southern margins of their range, along a depth gradient (E. radiata only), over a two year period (September 2010 – August 2012).

Identification info

Date (Creation): 2015-01-28

Citation identifier

doi:10.4226/77/57216ba85940c

Title: Information and documentation - Digital object identifier system

Date (Publication): 2016-04-28

Citation identifier: ISO 26324:2012

Citation identifier: https://doi.org/10.4226/77/57216ba85940c

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Flukes, Emma

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)

Private Bag 129

Hobart

Tasmania

7001

Australia

+61 (0)408901952

ORCID ID >

Collaborator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Johnson, Craig

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)

Private Bag 129

Hobart

Tasmania

7001

Australia

+61 3 6226 2582

ORCID ID >

Collaborator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Wright, Jeffrey

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)

Launceston

Tasmania

7250

Australia

+61 3 6324 3848

ORCID ID >

Purpose: To determine similarities and differences in the ecophysiology of temperate habitat-forming seaweeds across a depth and latitudinal gradient.

Credit: Australian Research Council (ARC) grant DP1096573

Status: Completed

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Flukes, Emma

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)

Private Bag 129

Hobart

Tasmania

7001

Australia

+61 (0)408901952

ORCID ID >

Temporal resolution: P0Y4M0DT0H0M0S

Topic category

Biota

Temporal extent

Time period: 2010-09-27 2012-08-07

Vertical element

Minimum value: 4

Maximum value: 31

Identifier: EPSG::5715

Name: MSL depth

Maintenance and update frequency: Not planned

Resource format

Title: CSV

Date

Edition: -

Keywords (Theme)

kelp
PAM fluorometry
ecophysiology
RNA:DNA
nucleic acid
fucoxanthin
mass spectrometry
HPLC
Relative electron transport rate (light-acclimated sample)
Alpha (light-acclimated sample)
Irradiance at which maximum electron rate occurs (light-acclimated sample)
Minimum saturating irradiance (light-acclimated sample)
Effective quantum yield (light-acclimated sample) - also ∆F/F'm
Relative electron transport rate (dark-acclimated sample)
Alpha (dark-acclimated sample)
Irradiance at which maximum electron rate occurs (dark-acclimated sample)
Minimum saturating irradiance (dark-acclimated sample)
Maximum quantum yield (dark-acclimated sample) - also Fv/Fm
Tissue concentration of fucoxanthin
Tissue concentration of chlorophyll a
Tissue concentration of chlorophyll c
Ratio of tissue RNA to DNA concentration
Tissue concentration of RNA
Tissue concentration of DNA
Tissue percentage carbon
Tissue percentage nitrogen
Ratio of tissue carbon to nitrogen
δ13C
δ15N

Keywords (Taxon)

Ecklonia radiata
Macrocystis pyrifera
Phyllospora comosa

NASA/GCMD Keywords, Version 8.5

Keywords (Discipline)

Temperate Reef

Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research

AODN Platform Vocabulary

diver

Resource constraints

Use limitation: The data described in this record are the intellectual property of the University of Tasmania through the Institute for Marine and Antarctic Studies.

Resource constraints

Classification: Unclassified

Resource constraints

Linkage: http://i.creativecommons.org/l/by/4.0/88x31.png
License Graphic

Title: Creative Commons Attribution 4.0 International License

Website: http://creativecommons.org/licenses/by/4.0/
License Text

Other constraints: Cite data as: Flukes, E.B., Johnson, C.R. & Wright, J.T. (2015). Ecophysiology of three habitat-forming seaweeds across a depth and latitudinal gradient in southeastern Australia. Institute for Marine and Antarctic Studies, University of Tasmania. doi:10.4226/77/57216ba85940c

Language: English

Character encoding: UTF8

Supplemental Information: Published citation TBA THESIS [soon to be available from UTAS e-collection]: Flukes, EB, Johnson, CR, Wright, JT (2015) Ecophysiology of habitat-forming seaweeds in a changing environment. PhD thesis, University of Tasmania.

Content Information

Content type: Physical measurement

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Relative electron transport rate (light-acclimated sample)

Name: L_rETRmax

Name: no units (relative units)

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Alpha (light-acclimated sample)

Name: L_Alpha

Name: nil

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Irradiance at which maximum electron rate occurs (light-acclimated sample)

Name: L_Em

Name: µmol photons m-2 s-1

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Minimum saturating irradiance (light-acclimated sample)

Name: L_Ek

Name: µmol photons m-2 s-1

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Effective quantum yield (light-acclimated sample) - also ∆F/F'm

Name: L_dFFm

Name: nil (ratio)

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Relative electron transport rate (dark-acclimated sample)

Name: D_rETRmax

Name: no units (relative units)

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Alpha (dark-acclimated sample)

Name: D_Alpha

Name: nil

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Irradiance at which maximum electron rate occurs (dark-acclimated sample)

Name: D_Em

Name: µmol photons m-2 s-1

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Minimum saturating irradiance (dark-acclimated sample)

Name: D_Ek

Name: µmol photons m-2 s-1

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Maximum quantum yield (dark-acclimated sample) - also Fv/Fm

Name: D_FvFm

Name: nil (ratio)

Description: measured using HPLC (note pigment concentrations not available for all sampling occasions)

Name: Tissue concentration of fucoxanthin

Name: Fucoxanthin

Name: mg g-1 wet weight

Description: measured using spectrophotometry (note pigment concentrations not available for all sampling occasions)

Name: Tissue concentration of chlorophyll a

Name: Chl_a

Name: mg g-1 wet weight

Description: measured using spectrophotometry (note pigment concentrations not available for all sampling occasions)

Name: Tissue concentration of chlorophyll c

Name: Chl_c

Name: mg g-1 wet weight

Description: Both RNA and DNA concentrations were extracted from a single sample of tissue

Name: Ratio of tissue RNA to DNA concentration

Name: RNA_DNA

Name: nil (ratio)

Description: measured using extraction method developed specifically for this data

Name: Tissue concentration of RNA

Name: RNA

Name: µg g wet weight-1

Description: measured using extraction method developed specifically for this data

Name: Tissue concentration of DNA

Name: DNA

Name: µg g wet weight-1

Description: measured using mass spectrometry

Name: Tissue percentage carbon

Name: percent_C

Name: %

Description: measured using mass spectrometry

Name: Tissue percentage nitrogen

Name: percent_N

Name: %

Description: measured using mass spectrometry

Name: Ratio of tissue carbon to nitrogen

Name: C_N

Name: nil (ratio)

Description: measured using mass spectrometry with Pee Dee Belemnite as standard

Name: δ13C

Name: d13C

Name: ‰

Description: measured using mass spectrometry with air as standard

Name: δ15N

Name: d15N

Name: ‰

Distribution Information

Distribution format

CSV (.csv)

OnLine resource: DATA ACCESS - seasonal, depth and ecophysiological measurements [direct download]

OnLine resource: imas:ARC_EFlukes_Ecophysiology_three_habitat_forming_seaweeds_se_australia
MAP - Sampling sites

OnLine resource: imas:ARC_EFlukes_Ecophysiology_three_habitat_forming_seaweeds_se_australia
This OCG WFS service returns the data for download in subsettable CSV format.

OnLine resource: View and download this data through the interactive IMAS Data Portal.

Resource lineage

Statement: Seaweeds were sampled quarterly in Tasmania, and every 6 months in NSW for 2 years (total of 8 sampling occasions for Tas and 4 for NSW). Ecklonia radiata was sampled at a depth of ~10 m and ~30 m, Phyllospora radiata was sampled at ~6 m, and Macrocystis pyrifera was sampled from the subsurface canopy (~0.5-1 m). Sampling consisted of PAM fluorometry measurements and collection of tissue samples for the following measurements: • Rapid Light Curves (light- and dark-adapted) using PAM fluorometry in situ - parameters are derived from fitted RLCs using the double exponential decay model of Platt et al (1980) • Pigment concentrations (chlorophyll a, c, and fucoxanthin) using HPLC and spectrophotometry) • Tissue chemistry (%C, %N, C:N, stable isotopes δ13C and δ15N) using a thermo gas chromatograph and isotope radio mass spectrometer • Nucleic acid characteristics (concentration of RNA and DNA, RNA:DNA) using a novel extraction technique developed specifically for brown macroalgae

Hierarchy level: Dataset

Resource lineage

Statement: A blue LED diving-PAM (Waltz) was used for all fluorometry. A Thermo Gas Chromatograph coupled to a Finnigan Mat Delta S isotope radio mass spectrometer operating in continuous flow mode was used for all tissue chemistry measurements. Fucoxanthin was measured using a Waters Acquity H-series UPLC™ coupled to a Waters Acquity Photodiode Array Detector with an Ethylene Bridged Hybrid (BEH) C-18 column (2.1 x 100 mm x 1.7 micron particles). Chlorophyll a and c were measured using a Dynamica HALO RB-10 Spectrophotometer.

Hierarchy level: Collection hardware

Hierarchy level: Dataset

Platform

Identifier

Code: diver

Metadata

Metadata identifier: urn:uuid/f5748c26-2fe9-47bd-b8bb-1e90c032de16

Language: English

Character encoding: UTF8

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - IMAS Data Manager

Type of resource

Resource scope: Dataset

Metadata linkage: https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/f5748c26-2fe9-47bd-b8bb-1e90c032de16
Point of truth URL of this metadata record

Date info (Creation): 2020-08-14T11:46:54

Date info (Revision): 2023-12-05T11:53:07

Metadata standard

Title: ISO 19115-3:2018

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