Date (Creation)

2015-01-28

Purpose

To determine similarities and differences in the ecophysiology of temperate habitat-forming seaweeds across a depth and latitudinal gradient.

Credit

Australian Research Council (ARC) grant DP1096573

Status

Completed

Temporal resolution

P0Y4M0DT0H0M0S

Topic category

• Biota

Maintenance and update frequency

Not planned

Keywords (Theme)

• kelp
• PAM fluorometry
• ecophysiology
• RNA:DNA
• nucleic acid
• fucoxanthin
• mass spectrometry
• HPLC

Keywords (Taxon)

• Ecklonia radiata
• Macrocystis pyrifera
• Phyllospora comosa

NASA/GCMD Keywords, Version 8.5

• EARTH SCIENCE | BIOSPHERE | VEGETATION | CHLOROPHYLL
• EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS)
• EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS) | BROWN ALGAE
• EARTH SCIENCE | BIOSPHERE | VEGETATION | PIGMENTS
• EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS
• EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | REEF
• EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | BENTHIC
• EARTH SCIENCE | BIOSPHERE | VEGETATION | NUTRIENTS

Keywords (Discipline)

• Temperate Reef

Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research

• Marine and Estuarine Ecology (incl. Marine Ichthyology)
• Phycology (incl. Marine Grasses)
• Ecological Physiology
• Ecological Impacts of Climate Change

AODN Platform Vocabulary

• diver

Language

English

Character encoding

UTF8

Supplemental Information

Published citation TBA THESIS [soon to be available from UTAS e-collection]: Flukes, EB, Johnson, CR, Wright, JT (2015) Ecophysiology of habitat-forming seaweeds in a changing environment. PhD thesis, University of Tasmania.

Content type

Physical measurement

Description

measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name

Relative electron transport rate (light-acclimated sample)

Name

L_rETRmax

Name

no units (relative units)

Description

measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name

Alpha (light-acclimated sample)

Name

L_Alpha

Name

nil

Description

measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name

Irradiance at which maximum electron rate occurs (light-acclimated sample)

Name

L_Em

Name

µmol photons m-2 s-1

Description

measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name

Minimum saturating irradiance (light-acclimated sample)

Name

L_Ek

Name

µmol photons m-2 s-1

Description

measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name

Effective quantum yield (light-acclimated sample) - also ∆F/F'm

Name

L_dFFm

Name

nil (ratio)

Description

measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name

Relative electron transport rate (dark-acclimated sample)

Name

D_rETRmax

Name

no units (relative units)

Description

measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name

Alpha (dark-acclimated sample)

Name

D_Alpha

Name

nil

Description

measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name

Irradiance at which maximum electron rate occurs (dark-acclimated sample)

Name

D_Em

Name

µmol photons m-2 s-1

Description

measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name

Minimum saturating irradiance (dark-acclimated sample)

Name

D_Ek

Name

µmol photons m-2 s-1

Description

measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name

Maximum quantum yield (dark-acclimated sample) - also Fv/Fm

Name

D_FvFm

Name

nil (ratio)

Description

measured using HPLC (note pigment concentrations not available for all sampling occasions)

Name

Tissue concentration of fucoxanthin

Name

Fucoxanthin

Name

mg g-1 wet weight

Description

measured using spectrophotometry (note pigment concentrations not available for all sampling occasions)

Name

Tissue concentration of chlorophyll a

Name

Chl_a

Name

mg g-1 wet weight

Description

measured using spectrophotometry (note pigment concentrations not available for all sampling occasions)

Name

Tissue concentration of chlorophyll c

Name

Chl_c

Name

mg g-1 wet weight

Description

Both RNA and DNA concentrations were extracted from a single sample of tissue

Name

Ratio of tissue RNA to DNA concentration

Name

RNA_DNA

Name

nil (ratio)

Description

measured using extraction method developed specifically for this data

Name

Tissue concentration of RNA

Name

RNA

Name

µg g wet weight-1

Description

measured using extraction method developed specifically for this data

Name

Tissue concentration of DNA

Name

DNA

Name

µg g wet weight-1

Description

measured using mass spectrometry

Name

Tissue percentage carbon

Name

percent_C

Name

%

Description

measured using mass spectrometry

Name

Tissue percentage nitrogen

Name

percent_N

Name

%

Description

measured using mass spectrometry

Name

Ratio of tissue carbon to nitrogen

Name

C_N

Name

nil (ratio)

Description

measured using mass spectrometry with Pee Dee Belemnite as standard

Name

δ13C

Name

d13C

Name

‰

Description

measured using mass spectrometry with air as standard

Name

δ15N

Name

d15N

Name

‰

Distribution format

• CSV (.csv)

OnLine resource

DATA ACCESS - seasonal, depth and ecophysiological measurements [direct download]

OnLine resource

imas:ARC_EFlukes_Ecophysiology_three_habitat_forming_seaweeds_se_australia

MAP - Sampling sites

OnLine resource

imas:ARC_EFlukes_Ecophysiology_three_habitat_forming_seaweeds_se_australia

This OCG WFS service returns the data for download in subsettable CSV format.

OnLine resource

View and download this data through the interactive IMAS Data Portal.

Statement

Seaweeds were sampled quarterly in Tasmania, and every 6 months in NSW for 2 years (total of 8 sampling occasions for Tas and 4 for NSW). Ecklonia radiata was sampled at a depth of ~10 m and ~30 m, Phyllospora radiata was sampled at ~6 m, and Macrocystis pyrifera was sampled from the subsurface canopy (~0.5-1 m). Sampling consisted of PAM fluorometry measurements and collection of tissue samples for the following measurements: • Rapid Light Curves (light- and dark-adapted) using PAM fluorometry in situ - parameters are derived from fitted RLCs using the double exponential decay model of Platt et al (1980) • Pigment concentrations (chlorophyll a, c, and fucoxanthin) using HPLC and spectrophotometry) • Tissue chemistry (%C, %N, C:N, stable isotopes δ13C and δ15N) using a thermo gas chromatograph and isotope radio mass spectrometer • Nucleic acid characteristics (concentration of RNA and DNA, RNA:DNA) using a novel extraction technique developed specifically for brown macroalgae

Hierarchy level

Dataset

Statement

A blue LED diving-PAM (Waltz) was used for all fluorometry. A Thermo Gas Chromatograph coupled to a Finnigan Mat Delta S isotope radio mass spectrometer operating in continuous flow mode was used for all tissue chemistry measurements. Fucoxanthin was measured using a Waters Acquity H-series UPLC™ coupled to a Waters Acquity Photodiode Array Detector with an Ethylene Bridged Hybrid (BEH) C-18 column (2.1 x 100 mm x 1.7 micron particles). Chlorophyll a and c were measured using a Dynamica HALO RB-10 Spectrophotometer.

Hierarchy level

Collection hardware

Resource scope

Dataset

Title

ISO 19115-3:2018