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Ecophysiology of three habitat-forming seaweeds across a depth and latitudinal gradient in southeastern Australia

Seasonal patterns in the in situ ecophysiology of the common habitat-forming seaweeds Ecklonia radiata, Phyllospora comosa, and Macrocystis pyrifera were investigated at different latitudes and depths in southeastern Australia. We used multiple performance indicators (photosynthetic characteristics, pigment content, chemical composition, stable isotopes, nucleic acids) to assess the ecophysiology of seaweeds near the northern and southern margins of their range, along a depth gradient (E. radiata only), over a two year period (September 2010 – August 2012).

Simple

Identification info

Date (Creation)
2015-01-28
Citation identifier
doi:10.4226/77/57216ba85940c

Title
Information and documentation - Digital object identifier system
Date (Publication)
2016-04-28
Citation identifier
ISO 26324:2012

Citation identifier
https://doi.org/10.4226/77/57216ba85940c

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Flukes, Emma
Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)
Private Bag 129
Hobart
Tasmania
7001
Australia
+61 (0)408901952
ORCID ID >

Collaborator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Johnson, Craig, Prof.
Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)
Private Bag 129
Hobart
Tasmania
7001
Australia
+61 3 6226 2582
ORCID ID >

Collaborator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Wright, Jeffrey, Dr
Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)
Launceston
Tasmania
7250
Australia
+61 3 6324 3848
ORCID ID >

Purpose
To determine similarities and differences in the ecophysiology of temperate habitat-forming seaweeds across a depth and latitudinal gradient.
Credit
Australian Research Council (ARC) grant DP1096573
Status
Completed

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Flukes, Emma, Dr
Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)
Private Bag 129
Hobart
Tasmania
7001
Australia
+61 (0)408901952
ORCID ID >

Temporal resolution
P0Y4M0DT0H0M0S
Topic category
  • Biota
N
S
E
W


N
S
E
W


Temporal extent

Time period
2010-09-27 2012-08-07

Vertical element

Minimum value
4
Maximum value
31
Identifier
EPSG::5715
Name
MSL depth
Maintenance and update frequency
Not planned

Resource format

Title
CSV
Date
Edition
-
Keywords (Theme)
  • kelp
  • PAM fluorometry
  • ecophysiology
  • RNA:DNA
  • nucleic acid
  • fucoxanthin
  • mass spectrometry
  • HPLC
Keywords (Taxon)
  • Ecklonia radiata
  • Macrocystis pyrifera
  • Phyllospora comosa
NASA/GCMD Keywords, Version 8.5
  • EARTH SCIENCE | BIOSPHERE | VEGETATION | CHLOROPHYLL
  • EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS)
  • EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS) | BROWN ALGAE
  • EARTH SCIENCE | BIOSPHERE | VEGETATION | PIGMENTS
  • EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS
  • EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | REEF
  • EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | BENTHIC
  • EARTH SCIENCE | BIOSPHERE | VEGETATION | NUTRIENTS
Keywords (Discipline)
  • Temperate Reef
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
  • Ecological Impacts of Climate Change
  • Marine and Estuarine Ecology (incl. Marine Ichthyology)
  • Phycology (incl. Marine Grasses)
  • Ecological Physiology
AODN Platform Vocabulary
  • diver
Keywords (Theme)
  • Relative electron transport rate (light-acclimated sample)
  • Alpha (light-acclimated sample)
  • Irradiance at which maximum electron rate occurs (light-acclimated sample)
  • Minimum saturating irradiance (light-acclimated sample)
  • Effective quantum yield (light-acclimated sample) - also ∆F/F'm
  • Relative electron transport rate (dark-acclimated sample)
  • Alpha (dark-acclimated sample)
  • Irradiance at which maximum electron rate occurs (dark-acclimated sample)
  • Minimum saturating irradiance (dark-acclimated sample)
  • Maximum quantum yield (dark-acclimated sample) - also Fv/Fm
  • Tissue concentration of fucoxanthin
  • Tissue concentration of chlorophyll a
  • Tissue concentration of chlorophyll c
  • Ratio of tissue RNA to DNA concentration
  • Tissue concentration of RNA
  • Tissue concentration of DNA
  • Tissue percentage carbon
  • Tissue percentage nitrogen
  • Ratio of tissue carbon to nitrogen
  • δ13C
  • δ15N

Resource constraints

Use limitation
The data described in this record are the intellectual property of the University of Tasmania through the Institute for Marine and Antarctic Studies.

Resource constraints

Classification
Unclassified

Resource constraints

Linkage
http://i.creativecommons.org/l/by/4.0/88x31.png

License Graphic

Title
Creative Commons Attribution 4.0 International License


>

Website
http://creativecommons.org/licenses/by/4.0/

License Text

Other constraints
Cite data as: Flukes, E.B., Johnson, C.R. & Wright, J.T. (2015). Ecophysiology of three habitat-forming seaweeds across a depth and latitudinal gradient in southeastern Australia. Institute for Marine and Antarctic Studies, University of Tasmania. doi:10.4226/77/57216ba85940c
Language
English
Character encoding
UTF8
Supplemental Information
Published citation TBA THESIS [soon to be available from UTAS e-collection]: Flukes, EB, Johnson, CR, Wright, JT (2015) Ecophysiology of habitat-forming seaweeds in a changing environment. PhD thesis, University of Tasmania.

Content Information

Content type
Physical measurement
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Relative electron transport rate (light-acclimated sample)

Name
L_rETRmax

Name
no units (relative units)
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Alpha (light-acclimated sample)

Name
L_Alpha

Name
nil
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Irradiance at which maximum electron rate occurs (light-acclimated sample)

Name
L_Em

Name
µmol photons m-2 s-1
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Minimum saturating irradiance (light-acclimated sample)

Name
L_Ek

Name
µmol photons m-2 s-1
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Effective quantum yield (light-acclimated sample) - also ∆F/F'm

Name
L_dFFm

Name
nil (ratio)
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Relative electron transport rate (dark-acclimated sample)

Name
D_rETRmax

Name
no units (relative units)
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Alpha (dark-acclimated sample)

Name
D_Alpha

Name
nil
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Irradiance at which maximum electron rate occurs (dark-acclimated sample)

Name
D_Em

Name
µmol photons m-2 s-1
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Minimum saturating irradiance (dark-acclimated sample)

Name
D_Ek

Name
µmol photons m-2 s-1
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Maximum quantum yield (dark-acclimated sample) - also Fv/Fm

Name
D_FvFm

Name
nil (ratio)
Description
measured using HPLC (note pigment concentrations not available for all sampling occasions)
Name
Tissue concentration of fucoxanthin

Name
Fucoxanthin

Name
mg g-1 wet weight
Description
measured using spectrophotometry (note pigment concentrations not available for all sampling occasions)
Name
Tissue concentration of chlorophyll a

Name
Chl_a

Name
mg g-1 wet weight
Description
measured using spectrophotometry (note pigment concentrations not available for all sampling occasions)
Name
Tissue concentration of chlorophyll c

Name
Chl_c

Name
mg g-1 wet weight
Description
Both RNA and DNA concentrations were extracted from a single sample of tissue
Name
Ratio of tissue RNA to DNA concentration

Name
RNA_DNA

Name
nil (ratio)
Description
measured using extraction method developed specifically for this data
Name
Tissue concentration of RNA

Name
RNA

Name
µg g wet weight-1
Description
measured using extraction method developed specifically for this data
Name
Tissue concentration of DNA

Name
DNA

Name
µg g wet weight-1
Description
measured using mass spectrometry
Name
Tissue percentage carbon

Name
percent_C

Name
%
Description
measured using mass spectrometry
Name
Tissue percentage nitrogen

Name
percent_N

Name
%
Description
measured using mass spectrometry
Name
Ratio of tissue carbon to nitrogen

Name
C_N

Name
nil (ratio)
Description
measured using mass spectrometry with Pee Dee Belemnite as standard
Name
δ13C

Name
d13C

Name
‰
Description
measured using mass spectrometry with air as standard
Name
δ15N

Name
d15N

Name
‰

Distribution Information

Distribution format
  • CSV (.csv)

OnLine resource
DATA ACCESS - seasonal, depth and ecophysiological measurements [direct download]

OnLine resource
imas:ARC_EFlukes_Ecophysiology_three_habitat_forming_seaweeds_se_australia

MAP - Sampling sites

OnLine resource
imas:ARC_EFlukes_Ecophysiology_three_habitat_forming_seaweeds_se_australia

This OCG WFS service returns the data for download in subsettable CSV format.

OnLine resource
View and download this data through the interactive IMAS Data Portal.

Resource lineage

Statement
Seaweeds were sampled quarterly in Tasmania, and every 6 months in NSW for 2 years (total of 8 sampling occasions for Tas and 4 for NSW). Ecklonia radiata was sampled at a depth of ~10 m and ~30 m, Phyllospora radiata was sampled at ~6 m, and Macrocystis pyrifera was sampled from the subsurface canopy (~0.5-1 m). Sampling consisted of PAM fluorometry measurements and collection of tissue samples for the following measurements: • Rapid Light Curves (light- and dark-adapted) using PAM fluorometry in situ - parameters are derived from fitted RLCs using the double exponential decay model of Platt et al (1980) • Pigment concentrations (chlorophyll a, c, and fucoxanthin) using HPLC and spectrophotometry) • Tissue chemistry (%C, %N, C:N, stable isotopes δ13C and δ15N) using a thermo gas chromatograph and isotope radio mass spectrometer • Nucleic acid characteristics (concentration of RNA and DNA, RNA:DNA) using a novel extraction technique developed specifically for brown macroalgae
Hierarchy level
Dataset

Resource lineage

Statement
A blue LED diving-PAM (Waltz) was used for all fluorometry. A Thermo Gas Chromatograph coupled to a Finnigan Mat Delta S isotope radio mass spectrometer operating in continuous flow mode was used for all tissue chemistry measurements. Fucoxanthin was measured using a Waters Acquity H-series UPLC™ coupled to a Waters Acquity Photodiode Array Detector with an Ethylene Bridged Hybrid (BEH) C-18 column (2.1 x 100 mm x 1.7 micron particles). Chlorophyll a and c were measured using a Dynamica HALO RB-10 Spectrophotometer.
Hierarchy level
Collection hardware
Hierarchy level
Dataset

Platform

Identifier

Code
diver

mdb:MD_Metadata

Metadata identifier
f5748c26-2fe9-47bd-b8bb-1e90c032de16

Language
English
Character encoding
UTF8

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - IMAS Data Manager

Type of resource

Resource scope
Dataset
Metadata linkage
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/f5748c26-2fe9-47bd-b8bb-1e90c032de16

Point of truth URL of this metadata record

Date info (Creation)
2020-08-14T11:46:54
Date info (Revision)
2020-08-14T11:46:54

Metadata standard

Title
ISO 19115-3:2018
 
 

Overviews

Spatial extent

N
S
E
W


N
S
E
W


Keywords

Alpha (dark-acclimated sample) Alpha (light-acclimated sample) Effective quantum yield (light-acclimated sample) - also ∆F/F'm HPLC Irradiance at which maximum electron rate occurs (dark-acclimated sample) Irradiance at which maximum electron rate occurs (light-acclimated sample) Maximum quantum yield (dark-acclimated sample) - also Fv/Fm Minimum saturating irradiance (dark-acclimated sample) Minimum saturating irradiance (light-acclimated sample) PAM fluorometry RNA:DNA Ratio of tissue RNA to DNA concentration Ratio of tissue carbon to nitrogen Relative electron transport rate (dark-acclimated sample) Relative electron transport rate (light-acclimated sample) Tissue concentration of DNA Tissue concentration of RNA Tissue concentration of chlorophyll a Tissue concentration of chlorophyll c Tissue concentration of fucoxanthin Tissue percentage carbon Tissue percentage nitrogen ecophysiology fucoxanthin kelp mass spectrometry nucleic acid δ13C δ15N
AODN Platform Vocabulary
diver
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
Ecological Impacts of Climate Change Ecological Physiology Marine and Estuarine Ecology (incl. Marine Ichthyology) Phycology (incl. Marine Grasses)
NASA/GCMD Keywords, Version 8.5
EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS) EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS) | BROWN ALGAE EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | BENTHIC EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | REEF EARTH SCIENCE | BIOSPHERE | VEGETATION | CHLOROPHYLL EARTH SCIENCE | BIOSPHERE | VEGETATION | NUTRIENTS EARTH SCIENCE | BIOSPHERE | VEGETATION | PIGMENTS

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Associated resources

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