Data for [The ISME Journal]: Microbial competition for iron determines its availability to the Ferrous Wheel

We dissect the spokes of the ferrous wheel associated with Fe demand by quantifying the uptake rates of heterotrophic bacteria and phytoplankton in the subantarctic Southern Ocean during summer and situate these findings within a seasonal context. To do so, we conducted bioassays in which the effects of light on Fe photochemistry and uptake physiology were studied by comparing light and dark incubations, and the effects of DOC supply and competition between phytoplankton and heterotrophic bacteria were examined by isolating bacteria from the larger members of the ferrous wheel by pre-incubation size fractionation.

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Identification info

Date (Publication)
2025-01-29T00:00:00

Identifier

Title
Information and documentation - Digital object identifier system
Citation identifier
ISO 26324:2012

Code
10.25959/9G97-VH65
Codespace
doi.org
Description
Digital Object Identifier (DOI)

Resource provider

Institute for Marine and Antarctic Studies
Private Bag 129
Hobart
Tasmania
7001
Australia

Principal investigator

Institute for Marine and Antarctic Studies - Strzepek, Robert
Private Bag 129
Hobart
TAS
7001
Australia
ROR ID >

ORCID >

Credit
Australian Research Council Special Research Initiative (Project Number SR200100008 (ACEAS) and ASCI000002 (AAPP)
Status
Completed

Point of contact

Institute for Marine and Antarctic Studies - Strzepek, Robert
Hobart
TAS
7001
Australia
ROR ID >

ORCID >

Topic category
  • Oceans

Extent

N
S
E
W


Temporal extent

Time period
2018-03-03 2021-01-16

Vertical element

Minimum value
0
Maximum value
70
Identifier
EPSG::5715
Name
MSL depth
Maintenance and update frequency
Not planned
Keywords (Theme)
  • Iron
  • carbon
  • Southern Ocean
  • microbes
  • heterotrophic bacteria
  • picoeukaryote
  • phytoplankton
  • competition
  • light
  • ferrous wheel
Global Change Master Directory Earth Science Keywords, Version 8.5
AODN Platform Vocabulary
AODN Geographic Extents Vocabulary

Resource constraints

Use limitation
Data, products and services from IMAS are provided "as is" without any warranty as to fitness for a particular purpose.

Resource constraints

Other constraints
This dataset is the intellectual property of the University of Tasmania (UTAS) through the Institute for Marine and Antarctic Studies (IMAS).

Resource constraints

Linkage
https://licensebuttons.net/l/by/4.0/88x31.png

License Graphic

Title
Creative Commons Attribution 4.0 International License
Alternate title
CC-BY
Edition
4.0


>

Website
https://creativecommons.org/licenses/by/4.0/

License Text

Other constraints
Cite data as: Strzepek, R. (2025). Data for [The ISME Journal]: Microbial competition for iron determines its availability to the Ferrous Wheel [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/9G97-VH65
Language
English
Character encoding
UTF8
Supplemental Information
Microbial competition for iron determines its availability to the Ferrous Wheel 10.1093/ismejo/wraf015

Content Information

Content type
Physical measurement

Identifier

Code
Iron uptake
Name
pmol L-1 d-1

Identifier

Code
Carbon uptake
Name
µmol L-1 d-1

Distribution Information

Distribution format
  • Microsoft Excel

OnLine resource
DATA ACCESS - Southern Ocean Fe uptake rates [.xlsx]

OnLine resource
ASSOCIATED PUBLICATION

Strzepek RF, Latour P, Ellwood MJ, Shaked Y, Boyd PW (2025). Microbial competition for iron determines its availability to the ferrous wheel. The ISME Journal. doi:10.1093/ismejo/wraf015

Resource lineage

Statement
Net primary productivity (i.e. inorganic C uptake) and Fe uptake rates were determined for water column samples collected at four to six depths between 0 and 70 m with the trace metal rosette. Water samples were collected pre-dawn at sampling depths determined from in situ PAR depth profiles obtained during midday CTD casts the day prior to collection. Samples were dispensed into 300 mL acid-washed polycarbonate bottles (ThermoScientific Nalgene, Waltham, MA, USA) and spiked with 16-20 μCi of sodium 14C-bicarbonate (NaH14CO3; specific activity 1.85 GBq mmol−1; PerkinElmer, Waltham, MA, USA) and 0.2 nmol L−1 of an acidified 55Fe solution (55FeCl3 in 0.1 M Ultrapure HCl; specific activity 30 MBq mmol−1; PerkinElmer). Six samples (five light and one “dark correction” bottle per irradiance) were incubated for 24h in a temperature-controlled deckboard incubator under natural sunlight at four to six light intensities (ranging from 67 to 1.0% of incident PAR). Light attenuation was adjusted by varying the layers of neutral density mesh and measured with a Biospherical Instruments QSL2101 Quantum Scalar PAR Sensor (San Diego, CA, USA). The incubation temperatures were 9, 10 and 12 ± 0.2°C for the spring, summer and fall experiments, respectively. After 24h of incubation, four replicate samples were serially vacuum-filtered (<10 mm Hg) through 20, 2.0, and 0.2 μm porosity polycarbonate filters (47 mm diameter; Poretics, Waltham, MA, USA) separated by 200 μm nylon mesh spacers to determine size-fractionated Fe uptake rates and NPP. Two size-fractionated samples were washed with Titanium (III) EDTA—citrate reagent for 5 min to remove extracellular Fe and rinsed three times with 15 mL of 0.2 μm-filtered seawater, and the other two size-fractionated samples were rinsed only with 0.2 μm-filtered seawater. In addition, two samples were filtered through 0.2 μm filters: an unfractionated light measurement and a dark correction bottle. The dark correction was 1.6 ± 1.4% of the C uptake rates measured in the light over multiple voyages, depths, and years (N=11). Data for the dark-corrected, size-fractionated samples rinsed with the Ti(III) EDTA— citrate reagent are reported here (i.e. intracellular Fe and C uptake) (N=2). The one exception to this protocol was for the size-fractionated depth profile samples collected from 19 m in summer 2020 that were performed in triplicate and incubated in parallel with the ferrous wheel experiment (N=3). Samples were counted on a liquid scintillation counter (PerkinElmer Tri-Carb 2910 TR) as described previously..
Hierarchy level
Dataset
Hierarchy level
Dataset

Platform

Identifier

Code
Investigator

Instrument

Identifier

Code
PerkinElmer Tri-Carb 2910 TR

Instrument

Identifier

Code
PerkinElmer Tri-Carb 2910 TR

Metadata

Metadata identifier
urn:uuid/d0a1067d-4348-467d-a8e7-7d8f57e24bab

Language
English
Character encoding
UTF8

Distributor

Institute for Marine and Antarctic Studies - (IMAS Data Manager)
IMAS website >

Type of resource

Resource scope
Dataset
Name
IMAS Dataset level record
Metadata linkage
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/d0a1067d-4348-467d-a8e7-7d8f57e24bab

Point of truth URL of this metadata record

Date info (Creation)
2025-01-29T00:00:00
Date info (Revision)
2025-01-29T00:00:00

Metadata standard

Title
ISO 19115-3:2018
 
 

Overviews

Spatial extent

N
S
E
W


Keywords

Iron Southern Ocean carbon competition ferrous wheel heterotrophic bacteria light microbes phytoplankton picoeukaryote
AODN Platform Vocabulary
Investigator
Global Change Master Directory Earth Science Keywords, Version 8.5
EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | BACTERIA/ARCHAEA EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | NUTRIENT CYCLING EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | SPECIES/POPULATION INTERACTIONS | SPECIES COMPETITION EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | AQUATIC ECOSYSTEMS | PLANKTON | PHYTOPLANKTON EARTH SCIENCE | OCEANS | OCEAN CHEMISTRY | BIOGEOCHEMICAL CYCLES EARTH SCIENCE | OCEANS | OCEAN CHEMISTRY | NUTRIENTS

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