IMAS Metadata Catalogue
RNA-seq data from Ecklonia radiata exposed to single and repeated marine heatwaves
This dataset contains raw RNA sequencing reads from juvenile Ecklonia radiata (Laminariales) collected from Bruny Island, Tasmania, and used in a controlled laboratory heatwave experiment. Individuals were exposed to ambient, single, or repeated marine heatwave treatments to assess the effects of thermal stress and recovery. Total RNA was extracted from blade tissue and sequenced on an Illumina platform to generate paired-end reads. The data represent high-quality raw FASTQ files (n = 30 samples; 5 replicates per treatment per time point) suitable for transcriptomic analyses examining the molecular responses of E. radiata to marine heatwaves.
Simple
Identification info
- Date (Publication)
- 2025-11-19T00:00:00
Identifier
- Title
- Information and documentation - Digital object identifier system
- Citation identifier
- ISO 26324:2012
- Code
- 10.25959/M4BS-SV29
- Codespace
- doi.org
- Description
- Digital Object Identifier (DOI)
- Credit
- This dataset was generated as part of Olivia J. Wynn’s PhD research at the Institute for Marine and Antarctic Studies (University of Tasmania) under the supervision of Professor Catriona L. Hurd, Dr. Damon Britton, and Dr. Cintia Iha, with collaborators Professor John Beardall and Assistant Professor Juan-Diego Gaitán-Espitia. This research was supported by the Australian Research Council (ARC) Discovery Project DP200101467, awarded to Professor Catriona L. Hurd.
- Status
- Completed
Author
- Topic category
-
- Biota
Extent
Temporal extent
- Time period
- 2024-07-30 2025-02-19
- Maintenance and update frequency
- Not planned
- Keywords (Taxon)
-
- Laminariales
- Global Change Master Directory Earth Science Keywords, Version 8.5
Resource constraints
- Use limitation
- Data, products and services from IMAS are provided "as is" without any warranty as to fitness for a particular purpose.
- Use limitation
- No known limitations.
Resource constraints
- Other constraints
- This dataset is the intellectual property of the University of Tasmania (UTAS) through the Institute for Marine and Antarctic Studies (IMAS).
Resource constraints
- Linkage
-
https://licensebuttons.net/l/by/4.0/88x31.png
License Graphic
- Title
- Creative Commons Attribution 4.0 International License
- Alternate title
- CC-BY
- Edition
- 4.0
- Website
-
https://creativecommons.org/licenses/by/4.0/
License Text
- Other constraints
- Cite data as: Wynn, O. (2025). RNA-seq data from Ecklonia radiata exposed to single and repeated marine heatwaves [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/M4BS-SV29
- Language
- English
- Character encoding
- UTF8
Content Information
- Content type
- Physical measurement
Identifier
- Code
- RNA sequencing reads
- Name
- Raw paired-end reads (FASTQ format)
- Name
- Base pairs (bp)
Distribution Information
- Distribution format
-
- FASTQ
- OnLine resource
- DATA ACCESS - browse and download available files
Resource lineage
- Statement
- Juvenile Ecklonia radiata sporophytes were collected from Bruny Island, Tasmania, and acclimated in laboratory aquaria under controlled conditions. Individuals were exposed to three experimental temperature regimes simulating marine heatwave scenarios: no heatwave (15 °C throughout), a single 6-day heatwave at 20 °C, or two consecutive 6-day heatwaves at 20 °C separated by recovery periods at 15 °C. Samples for RNA sequencing were collected at two time points, during heatwave exposure and following recovery, from each treatment (n = 5 per treatment per time point). Blade tissue (approximately 50 mg) from each individual was preserved in RNA stabilization buffer and stored at –20 °C prior to extraction. Total RNA was extracted and purified before library preparation using the Illumina TruSeq Stranded mRNA Kit. Sequencing was performed by Macrogen Oceania (Sydney, Australia) on an Illumina NovaSeq X platform, generating 150 bp paired-end reads. This dataset contains the resulting raw FASTQ files for all samples.
- Hierarchy level
- Dataset
- Hierarchy level
- Dataset
Platform
Identifier
- Code
- High-throughput sequencing
Instrument
Identifier
- Code
- Illumina NovaSeq X
Metadata
- Metadata identifier
- urn:uuid/a9fc13db-1292-4e8f-a5f0-cde07af6ccd8
- Language
- English
- Character encoding
- UTF8
Type of resource
- Resource scope
- Dataset
- Name
- IMAS Dataset level record
- Metadata linkage
-
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/a9fc13db-1292-4e8f-a5f0-cde07af6ccd8
Point of truth URL of this metadata record
- Date info (Creation)
- 2025-11-10T00:00:00
- Date info (Revision)
- 2025-11-19T20:21:21
Metadata standard
- Title
- ISO 19115-3:2018
Overviews
Spatial extent
Provided by
Associated resources
Not available