IMAS Metadata Catalogue
RNA-seq data from Ecklonia radiata exposed to combined temperature and CO₂ treatments
This dataset contains raw RNA sequencing reads from juvenile Ecklonia radiata (Laminariales) collected from Coal Point, Tasmania, and used in a controlled laboratory experiment examining the effects of temperature and elevated CO₂ on gene expression. Individuals were exposed to three temperatures (6, 16, and 26 °C) under two CO₂ levels: current (~420 µatm) and future (~1000 µatm), representing RCP 8.5 projections. Total RNA was extracted from blade tissue and sequenced on an Illumina NovaSeq 6000 platform to produce 150 bp paired-end reads. The dataset includes raw FASTQ files from 30 biological samples (n = 5 per treatment combination) used for transcriptomic analyses investigating the molecular responses of E. radiata to ocean warming and acidification.
Simple
Identification info
- Date (Publication)
- 2025-11-19T00:00:00
Identifier
- Title
- Information and documentation - Digital object identifier system
- Citation identifier
- ISO 26324:2012
- Code
- 10.25959/QZXM-VV84
- Codespace
- doi.org
- Description
- Digital Object Identifier (DOI)
- Credit
- This dataset was generated as part of Olivia J. Wynn’s PhD research at the Institute for Marine and Antarctic Studies (University of Tasmania) under the supervision of Professor Catriona L. Hurd, Dr Damon Britton, and Dr Cintia Iha, with collaborators Professor John Beardall and Assistant Professor Juan-Diego Gaitán-Espitia. This research was supported by the Australian Research Council (ARC) Discovery Project DP200101467, awarded to Professor Catriona L. Hurd.
- Status
- Completed
Author
- Topic category
-
- Biota
Extent
Temporal extent
- Time period
- 2021-02-22 2022-05-17
- Maintenance and update frequency
- Not planned
- Keywords (Taxon)
-
- Laminariales
- Global Change Master Directory Earth Science Keywords, Version 8.5
Resource constraints
- Use limitation
- Data, products and services from IMAS are provided "as is" without any warranty as to fitness for a particular purpose.
- Use limitation
- No known limitations.
Resource constraints
- Other constraints
- This dataset is the intellectual property of the University of Tasmania (UTAS) through the Institute for Marine and Antarctic Studies (IMAS).
Resource constraints
- Linkage
-
https://licensebuttons.net/l/by/4.0/88x31.png
License Graphic
- Title
- Creative Commons Attribution 4.0 International License
- Alternate title
- CC-BY
- Edition
- 4.0
- Website
-
https://creativecommons.org/licenses/by/4.0/
License Text
- Other constraints
- Cite data as: Wynn, O. (2025). RNA-seq data from Ecklonia radiata exposed to combined temperature and CO₂ treatments [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/QZXM-VV84
- Language
- English
- Character encoding
- UTF8
Content Information
- Content type
- Physical measurement
Identifier
- Code
- RNA sequencing reads
- Name
- Raw paired-end reads (FASTQ format)
- Name
- Base pairs (bp)
Distribution Information
- Distribution format
-
- FASTQ
- OnLine resource
- DATA ACCESS - browse and download available files
Resource lineage
- Statement
- Juvenile Ecklonia radiata were collected from Coal Point, Tasmania, and acclimated under controlled laboratory conditions. Individuals were exposed to a factorial design of three temperatures (6, 16, 26 °C) and two CO₂ levels (~420 µatm and ~1000 µatm) for 21 days. Blade tissue (~50 mg) was preserved in RNA stabilization buffer and stored at −20 °C prior to extraction. Total RNA was extracted, purified, and prepared for sequencing using the Illumina TruSeq RNA Library Prep Kit with poly(A) enrichment. Sequencing was performed by Macrogen Inc. (Seoul, South Korea) on an Illumina NovaSeq 6000, generating 150 bp paired-end reads. This dataset includes the resulting raw FASTQ files for all samples.
- Hierarchy level
- Dataset
- Hierarchy level
- Dataset
Platform
Identifier
- Code
- High-throughput sequencing
Instrument
Identifier
- Code
- Illumina NovaSeq 6000
Metadata
- Metadata identifier
- urn:uuid/8a3b5597-32d4-4b9d-9284-939b6197ded5
- Language
- English
- Character encoding
- UTF8
Type of resource
- Resource scope
- Dataset
- Name
- IMAS Dataset level record
- Metadata linkage
-
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/8a3b5597-32d4-4b9d-9284-939b6197ded5
Point of truth URL of this metadata record
- Date info (Creation)
- 2025-11-10T00:00:00
- Date info (Revision)
- 2025-11-19T20:21:23
Metadata standard
- Title
- ISO 19115-3:2018
Overviews
Spatial extent
Provided by
Associated resources
Not available