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PCR genetic screening for presence of sea urchin DNA in rock lobster faecal samples

Data is PCR amplification results of southern rock lobster (Jasus edwardsii) faecal material tested for sea urchin DNA (using unique primers for Centrostephanus rodgersii and Heliocidaris erythrogramma) in an attempt to determine in situ rates of consumption of sea urchins by lobsters. An efficient and non-lethal method was used to source and screen lobster faecal samples for the presence of DNA from ecologically important sea urchins. Lobster faecal samples were collected from trap caught specimens sourced in winter & summer seasons over 2 years (2009-2011) within two no-take research reserves; declared specifically for the purpose of rebuilding large predatory-capable lobsters to assess the potential for predator-driven remediation of kelp beds on rocky reefs extensively overgrazed by sea urchins (North Eastern Tasmania) and reefs showing initial signs of overgrazing (South Eastern Tasmania). Data for molecular assays showed high variability in the proportion of lobsters testing positive to sea urchins, with significant variability detected across different years and seasons but this was found to vary depending on different lobster size-classes. Sea urchin DNA was also amplifiable from sediments and urchin faeces collected from the reef surface where urchins occurred in high abundance. Furthermore, positive sea urchin DNA assays were obtainable from lobster faeces after lobsteres were fed sediment and urchin faecal material.


Rates of predation obtained with genetics tests can also be compared to independent rates of urchin losses given known lobster abundances within research reserves (and at control sites). Data of changes in urchin abundances and lobster abundances are therefore also lodged as part of this record.

Simple

Identification info

Date (Creation)
2011-09-16T15:58:00

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Johnson, Craig, Prof.
IMAS-Sandy Bay
Private Bag 129
Hobart
TAS
7001
Australia
61 3 6226 2582
61 3 6226 2973 (facsimile)
ORCID ID >

Collaborator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Ling, Scott, Dr (Research Associate)
IMAS-Sandy Bay
Private Bag 129
Hobart
TAS
7001
Australia
61 3 6226 2619
61 3 6226 2973 (facsimile)
ORCID ID >

Collaborator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Redd, Kevin (PhD Student)
IMAS-Sandy Bay
Private Bag 129
Hobart
TAS
7001
Australia
Purpose
Assessment of Management Options to Minimise Formation of 'Barrens' Habitat by the Long-Spined Sea Urchin (Centrostephanus rodgersii) in Tasmania
Credit
Funding: FRDC project 2007/045
Status
Completed

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Johnson, Craig, Prof.
IMAS - Sandy Bay
Private Bag 129
Hobart
TAS
7001
Australia
61 3 6226 2582
61 3 6226 2973 (facsimile)
ORCID ID >

Collaborator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Ling, Scott, Dr (Marine Experimental and Quantitative Ecologist)
IMAS - Sandy Bay
Private Bag 129
Hobart
TAS
7001
Australia
61 3 6226 2619
61 3 6226 2973 (facsimile)
ORCID ID >

Spatial representation type
Text, table
Temporal resolution
P0Y6M0DT0H0M0S
Topic category
  • Biota

Extent

N
S
E
W


N
S
E
W


Temporal extent

Time period
2008-04-01T00:00:00 2011-02-28T00:00:00

Vertical element

Minimum value
3
Maximum value
40
Identifier
EPSG::5715
Name
MSL depth
Maintenance and update frequency
Not planned

Resource format

Title
Microsoft Excel (xlsx)
Date
Edition
2007
Keywords (Taxon)
  • Centrostephanus rodgersii
  • Jasus edwardsii
  • Haliotis rubra
  • Ecklonia radiata
  • Heliocidaris erythrogramma
AODN Keywords Thesaurus
  • Genetics
  • Urchin Barrens
  • Kelp Beds
  • DNA Detection
Global Change Master Directory (GCMD) Earth Science Keywords Version 8.0
  • ANIMALS/INVERTEBRATES
  • REEF HABITAT
  • TROPHIC DYNAMICS
  • COMMUNITY STRUCTURE
  • MARINE HABITAT
  • ECHINODERMS
  • CRUSTACEANS
  • SEA URCHINS
Keywords (Discipline)
  • Temperate Reef
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
  • Fish Physiology and Genetics
  • Marine and Estuarine Ecology (incl. Marine Ichthyology)

Resource constraints

Classification
Restricted

Resource constraints

Use limitation
The data described in this record are the intellectual property of University of Tasmania through the Institute for Marine and Antarctic Studies

Resource constraints

Linkage
http://i.creativecommons.org/l/by-sa/2.5/au/88x31.png

License Graphic

Title
Creative Commons Attribution-Share Alike 2.5 Australia License


>

Website
http://creativecommons.org/licenses/by-sa/2.5/au/

License Text

Other constraints
The citation in a list of references is: citation author name/s (year metadata published), metadata title. Citation author organisation/s. File identifier and Data accessed at (add http link).
Other constraints
See point of contact for access to the data.
Other constraints
Use of this data is currently embargoed until publication in the peer-review literature.

Associated resource

Title
FRDC project 2007/045
Date (unknown)
2011-09-16T00:00:00
Language
English
Character encoding
UTF8
Supplemental Information
References: Final FRDC Report in draft format.

Distribution Information

Distribution format
  • Microsoft Excel (xlsx)

OnLine resource
DATA - Genetic tests on lobster faeces [direct download]

OnLine resource
DATA - Genetic tests of sea urchin DNA in environmental samples [direct download]

OnLine resource
DATA - Genetic tests of sea urchin faecal samples [direct download]

OnLine resource
DATA - Genetic tests for sea urchin DNA in lobster faeces fed environmental and sea urchin faecal samples [direct download]

OnLine resource
DATA - Sea urchin density change within Research Reserves [direct download]

OnLine resource
DATA - Sea urchin density change within Research Reserves relative to control sites [direct download]

OnLine resource
README - data file help document [direct download]

OnLine resource
imas:FR2_CRJohnson_Presence_sea_urchin_dna_in_rock_lobster_faecal_samples_GV

MAP - Collection sites for lobster faecal samples

Resource lineage

Statement
PCR detection of urchin DNA in lobster faecal material. Refer to "Data file help document” for general methods information, and for a full description of methods see the Draft Final Report of the Fisheries Research & Development Corporation research project #FRDC2007_045.
Hierarchy level
Dataset

Resource lineage

Statement
GPS used datum WGS84.
Hierarchy level
Collection hardware

Metadata

Metadata identifier
665c701d-0737-4093-8ba0-93765fc12c94

Language
English
Character encoding
UTF8

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - IMAS Data Manager

Type of resource

Resource scope
Dataset
Metadata linkage
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/665c701d-0737-4093-8ba0-93765fc12c94

Point of truth URL of this metadata record

Date info (Creation)
2020-09-17T17:43:27
Date info (Revision)
2020-09-17T17:43:27

Metadata standard

Title
ISO 19115-3:2018
 
 

Overviews

Spatial extent

N
S
E
W


N
S
E
W


Keywords

AODN Keywords Thesaurus
DNA Detection Genetics Kelp Beds Urchin Barrens
Global Change Master Directory (GCMD) Earth Science Keywords Version 8.0
ANIMALS/INVERTEBRATES COMMUNITY STRUCTURE CRUSTACEANS ECHINODERMS MARINE HABITAT REEF HABITAT SEA URCHINS TROPHIC DYNAMICS

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Access to the record in catalogue
Read here the full details and access to the data.

Associated resources

Not available


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