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Growth rate and Fast Repetition Rate fluorometry (FRRf) of phytoplankton

Ocean alkalinity enhancement (OAE) is a promising carbon removal method, but it may cause a significant perturbation of the ocean with trace metals such as Nickel (Ni). This study tested the effect of increasing Ni concentrations on phytoplankton growth and photosynthesis. The data were the growth rates of 11 phytoplankton species under different Ni concentrations (Master thesis project). The growth rates were calculated using daily fluorescence signal values measured by the fluorometer. Fv/Fm and SigmaPSII data were measured using Fast repetition rate fluorometry (FRRf). The growth rate and photo-physiological response of phytoplankton was analysed using generalised additive models (GAMs) and plotted in RStudio (R packages “mgcv” and “ggplot2”).

Simple

Identification info

Date (Creation)
2021-11-19
Citation identifier
doi:10.25959/1Z63-7555

Title
Information and documentation - Digital object identifier system
Date (Publication)
2021-11-25T00:00:00
Citation identifier
ISO 26324:2012

Owner

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Guo, Jiaying
IMAS - Hobart
Private Bag 129
Hobart
Tasmania
Hobart
Australia
Credit
This study was funded by the Australian Research Council by Future Fellowship FT200100846 awarded to Lennart Bach.
Status
complete

Owner

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Guo, Jiaying
IMAS - Hobart
Private Bag 129
Hobart
Tasmania
7001
Australia

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Bach, Lennart
IMAS - Hobart
Private Bag 129
Hobart
Tasmania
7001
Australia
Temporal resolution
P0Y0M1DT0H0M0S
Topic category
  • Biota

Extent

Temporal extent

Time period
2021-03-01 2021-08-31
Maintenance and update frequency
none-planned

Resource format

Date
NASA/GCMD Keywords, Version 8.5
  • EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PLANTS | MICROALGAE
  • EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | BACTERIA/ARCHAEA | CYANOBACTERIA (BLUE-GREEN ALGAE)
  • EARTH SCIENCE | TERRESTRIAL HYDROSPHERE | WATER QUALITY/WATER CHEMISTRY | TRACE METALS
Keywords (Theme)
  • Growth rate
  • total dissolved nickel concentrations
  • pNi
  • free nickel ion concentrations
  • negtively log10-transforred free nickel ion concentrations
  • Minimum ST-ChlF in the dark-regulated state
  • Maximum ST-ChlF in the dark-regulated state
  • the maximum quantum yield
  • the functional absorption cross-section of photosystem II (σPSII)
  • re- opening of closed reaction center II (RCII)
  • Estimated degrees of freedom for the model terms
  • estimated residual degrees of freedom
  • Simply a ratio of two variances
  • number of basis functions
  • k-index
  • Adjusted r squred
  • 95% confidence interval
  • Deviance explained
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
  • Biological Oceanography

Resource constraints

Use limitation
The data described in this record are the intellectual property of the University of Tasmania through the Institute for Marine and Antarctic Studies.

Resource constraints

Classification
Unclassified

Resource constraints

Linkage
http://i.creativecommons.org/l/by/4.0/88x31.png

License Graphic

Title
Creative Commons Attribution 4.0 International License


>

Website
http://creativecommons.org/licenses/by/4.0/

License Text

Other constraints
Cite data as: Guo, J. (2021). Growth rate and Fast Repetition Rate fluorometry (FRRf) of phytoplankton [Data set]. Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS). https://doi.org/10.25959/1Z63-7555
Language
English
Character encoding
UTF8

Content Information

Content type
Physical measurement
Name
Growth rate

Name
Growth_rate

Name
day-1
Name
total dissolved nickel concentrations

Name
Ni

Name
mol/L
Name
pNi

Name
negtively log10-transforred total dissolved nickel concentrations

Name
NBS
Name
free nickel ion concentrations

Name
NI2+

Name
mol/L
Name
negtively log10-transforred free nickel ion concentrations

Name
pNi2+

Name
NBS
Name
Minimum ST-ChlF in the dark-regulated state

Name
Fo

Name
arbitrary units
Name
Maximum ST-ChlF in the dark-regulated state

Name
Fm

Name
arbitrary units
Name
the maximum quantum yield

Name
Fv/Fm

Name
no units
Name
the functional absorption cross-section of photosystem II (σPSII)

Name
Sigma

Name
nm2 reaction centre (RC)-1
Name
re- opening of closed reaction center II (RCII)

Name
TauES

Name
μs
Name
Estimated degrees of freedom for the model terms

Name
edf

Name
no unit
Name
estimated residual degrees of freedom

Name
Ref.df

Name
no unit
Name
Simply a ratio of two variances

Name
F

Name
no unit
Name
number of basis functions

Name
k'

Name
no unit
Name
k-index

Name
kindex

Name
no unit
Name
Adjusted r squred

Name
adjR2

Name
no unit
Name
95% confidence interval

Name
95%

Name
no unit
Name
Deviance explained

Name
DE

Name
no unit

Distribution Information

Distribution format
  • Microsoft excel

OnLine resource
DATA ACCESS - growth rate and fluorometry data [direct file download]

Resource lineage

Statement
Phytoplankton cultures were kept in tubes and grown at 17°C. The light was provided on a 14-to-10-hour daily cycle (cool white fluorescence light) where light intensities were 58 μmol photons m-2 s-1 (14 hours) and 23 μmol photons m-2 s-1 (10 hours). The fluorescence of these cultures was measured daily using a fluorometer and values during the exponential growth phase were ln-transferred for growth rate calculation. Fv/Fm and SigmaPSII data were measured using Fast repetition rate fluorometry (FRRf). The growth rate and photo-physiological response of phytoplankton were analysed using generalised additive models (GAMs) and plotted in RStudio (R packages “mgcv” and “ggplot2”).
Hierarchy level
Dataset

Metadata

Metadata identifier
5aa2f15d-cac9-4b6c-9955-ddcfd61f8534

Language
English
Character encoding
UTF8

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - IMAS Data Manager ()

Type of resource

Resource scope
Dataset
Metadata linkage
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/5aa2f15d-cac9-4b6c-9955-ddcfd61f8534

Point of truth URL of this metadata record

Date info (Creation)
2015-05-06T11:44:25
Date info (Revision)
2015-05-06T11:44:25

Metadata standard

Title
ISO 19115-3:2018
 
 

Overviews

Spatial extent

Keywords

95% confidence interval Adjusted r squred Deviance explained Estimated degrees of freedom for the model terms Growth rate Maximum ST-ChlF in the dark-regulated state Minimum ST-ChlF in the dark-regulated state Simply a ratio of two variances estimated residual degrees of freedom free nickel ion concentrations k-index negtively log10-transforred free nickel ion concentrations number of basis functions pNi re- opening of closed reaction center II (RCII) the functional absorption cross-section of photosystem II (σPSII) the maximum quantum yield total dissolved nickel concentrations
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
Biological Oceanography
NASA/GCMD Keywords, Version 8.5
EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | BACTERIA/ARCHAEA | CYANOBACTERIA (BLUE-GREEN ALGAE) EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PLANTS | MICROALGAE EARTH SCIENCE | TERRESTRIAL HYDROSPHERE | WATER QUALITY/WATER CHEMISTRY | TRACE METALS

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Associated resources

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