This record provides an overview of the NESP Marine and Coastal Hub Emerging Priorities project "Learning from the field: a rapid assessment of the composition and ecotoxicology of the harmful algal bloom in South Australia". For specific data outputs from this project, please see child records associated with this metadata.

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A severe harmful algal bloom (HAB) involving Karenia species has affected large areas of the South Australian coastline, including Gulf St Vincent, Spencer Gulf and Investigator Strait. The species composition, toxicity and ecological impact pathways of the bloom remain poorly understood, limiting the ability of agencies to respond to ongoing and future bloom events.

This Emerging Priorities project will conduct a rapid investigation of the composition, toxicity and variability of the South Australian Karenia bloom across sites, depths and bloom stages. Broadscale water sampling will target areas of bloom spread and prolonged exposure, with sampling locations adjusted using satellite chlorophyll maps and field observations.

The project will combine microscopy, qPCR-based species identification and abundance estimates, rotifer bioassays, and brevetoxin screening. Rotifer assays will provide a rapid comparative indicator of aquatic toxicity, while qPCR will identify and quantify Karenia species in the same samples, allowing toxicity results to be linked with species composition and bloom development. Selected field samples and cultured strains will also be screened for brevetoxins in collaboration with allied HAB research initiatives.

Outputs will include baseline information on bloom toxicity and variability, qPCR-linked species composition data, a rapid rotifer assay method, and a foundational dataset linking Karenia community structure with toxicological response. The findings will support SA DEW, Commonwealth agencies and other research users in understanding bloom impact pathways, improving future HAB response, and informing long-term monitoring and marine environmental management.

Outputs

• Integrated harmful algal bloom field, water-quality and toxicology dataset across Gulf St Vincent, Spencer Gulf and Investigator Strait [dataset]

• Karenia species composition and abundance dataset derived from microscopy and qPCR analyses [dataset]

• Toxicity assessment dataset incorporating rotifer bioassays and brevetoxin screening results [dataset]

• Final technical report [written]