SO298 Ocean Alkalinity Enhancement (OAE) Incubation

To investigate how the unavoidable physical and chemical perturbations associated with Ocean Alkalinity Enhancement (OAE) could influence marine plankton communities and how potential side-effects compare to impacts of climate change, we conducted 19 ship-based experiments in the Equatorial Pacific, examining three prevalent OAE source (NaOH, olivine, and steel slag) and their impacts on natural phytoplankton populations. Our experiments simulated realistic and moderate alkalinity enhancements between 29-16 μmol kg-1. The monitored parameters included total chlorophyll-a concentrations, macro nutrients, trace elements, total alkalinity, Fv/Fm, pH,and flowcytometry.

Identification info

Date (Publication): 2024-06-25T00:00:00

Citation identifier

doi:10.25959/BMV8-1K07

Title: Information and documentation - Digital object identifier system

Date (Publication): 2024-06-25

Citation identifier: ISO 26324:2012

Citation identifier: https://doi.org/10.25959/BMV8-1K07

Resource provider

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)

Private Bag 129

Hobart

Tasmania

7001

Australia

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Guo, Jiaying

Private Bag 129

Hobart

TAS

7001

Australia

ORCID ID >

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Bach, Lennart

Private Bag 129

Hobart

TAS

7001

Australia

ORCID ID >

Status: Completed

Author

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Guo, Jiaying

Hobart

TAS

7001

Australia

ORCID ID >

Owner

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Bach, Lennart

Hobart

TAS

7001

Australia

ORCID ID >

Topic category

Biota

Extent

Temporal extent

Time period: 2023-04-14 2023-06-02

Maintenance and update frequency: Not planned

Keywords (Theme)

Ocean Alkalinity Enhancement

Global Change Master Directory Earth Science Keywords, Version 8.5

AODN Discovery Parameter Vocabulary

Concentration of chlorophyll-a per unit volume of the water body
Total alkalinity per unit mass of the water body
Concentration of phosphate {PO4} per unit mass of the water body
Concentration of silicate {SiO4} per unit mass of the water body
Concentration of nitrate and nitrite {NO3 and NO2} per unit mass of the water body
the maximum potential quantum efficiency of Photosystem II
pH (total scale) of the water body
Abundance of biota
EXP

AODN Geographic Extents Vocabulary

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Use limitation: Data, products and services from IMAS are provided "as is" without any warranty as to fitness for a particular purpose.

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Other constraints: This dataset is the intellectual property of the University of Tasmania (UTAS) through the Institute for Marine and Antarctic Studies (IMAS).

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Linkage: https://licensebuttons.net/l/by-nc/4.0/88x31.png
License Graphic

Title: Creative Commons Attribution-NonCommercial 4.0 International License

Alternate title: CC-BY-NC

Edition: 4.0

Website: https://creativecommons.org/licenses/by-nc/4.0/
License Text

Other constraints: Cite data as: Guo, J., & Bach, L. (2024). SO298 Ocean Alkalinity Enhancement (OAE) Incubation [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/BMV8-1K07

Language: English

Character encoding: UTF8

Content Information

Content type: Physical measurement

Identifier

Code: Concentration of chlorophyll-a per unit volume of the water body

Identifier: http://vocab.nerc.ac.uk/collection/P06/current/UGPL

Name: Micrograms per litre

Identifier

Code: Total alkalinity per unit mass of the water body

Identifier: http://vocab.nerc.ac.uk/collection/P06/current/KGUM

Name: Micromoles per kilogram

Identifier

Code: Concentration of phosphate {PO4} per unit mass of the water body

Identifier: http://vocab.nerc.ac.uk/collection/P06/current/UPOX

Name: Micromoles per litre

Identifier

Code: Concentration of silicate {SiO4} per unit mass of the water body

Identifier: http://vocab.nerc.ac.uk/collection/P06/current/UPOX

Name: Micromoles per litre

Identifier

Code: Concentration of nitrate and nitrite {NO3 and NO2} per unit mass of the water body

Identifier: http://vocab.nerc.ac.uk/collection/P06/current/UPOX

Name: Micromoles per litre

Identifier

Code: the maximum potential quantum efficiency of Photosystem II

Name: Fv/Fm

Identifier: http://vocab.nerc.ac.uk/collection/P06/current/UUUU

Name: Dimensionless

Identifier

Code: pH (total scale) of the water body

Identifier: http://vocab.nerc.ac.uk/collection/P06/current/UUPH

Name: pH units

Identifier

Code: Abundance of biota

Identifier: http://vocab.nerc.ac.uk/collection/P06/current/UPMM

Name: Number per cubic metre

Identifier

Code: EXP

Name: experiment number

Identifier: http://vocab.nerc.ac.uk/collection/P06/current/UUUU

Name: Dimensionless

Distribution Information

Distribution format

OnLine resource: DATA ACCESS - Download OAE incubation data [.zip]

Resource lineage

Statement: Incubation experiments setup Nineteen incubation experiments were undertaken to assess the impacts of NaOH- olivine- and slag-OAE on phytoplankton growth and community composition. For each experiment, twelve acid-washed 500 mL polycarbonate bottles (Nalgene) were filled with surface seawater collected with a trace metal clean towed water sampling device (so called ‘tow-fish’) after sunset at ~2 m depth. The collection of seawater was conducted in a trace metal-clean plastic tent (the ‘bubble’) that was over-pressurised with HEPA-filtered air to minimize trace metal contamination. Three bottles were left unperturbed and sampled immediately to determine the initial conditions (see below). Three bottles were supplemented with 150 µL of 0.1M NaOH (Analytical grade); three with 0.2 g of ground olivine powder; three with 0.001g of slag powder; and three remained unperturbed as control. These bottles were incubated for 48 h in an on-deck incubator flushed continuously with surface seawater and screened to receive ca. 35% of surface incident light. Sampling protocol Initial seawater was stored in dark HDPE bottles before measurements. The pH was carefully measured using a pH meter (914 pH/Conductometer Metrohm), following the procedural reference. Eight mL of sample was filtered (0.2 µm) for macronutrient analysis using a QUAATRO39 (Seal Analytical) autoanalyzer directly after sampling. Alkalinity samples (60 mL) of the control and the NaOH treatment were fixed with 30 µL HgCl2 and stored for 3 months until analysis. Alkalinity samples of the olivine and slag treatments were filtered after sampling (0.22 µm PES syringe filters) to remove remaining particles. In Experiments 3,8 and 9, certain replicate bottles from both the treatment and control groups exhibited lower alkalinity, which could be due to rainwater dilution effects that may have occurred in a patchy manner during seawater collection with the tow-fish. Therefore, we compared the high and low alkalinity samples in the treatment group with the corresponding high and low alkalinity samples in the control group to determine the ∆ alkalinity values in these specific experiments. After 48 h, experimental bottles were removed from incubators, samples were transferred into dark bottles in the ‘bubble’. Like the initial samples, the following samples were subsampled from the dark bottles from each treatment: pH, flow cytometry (2 mL for phytoplankton and 1 mL for bacteria samples), Chl-a (100 mL), FRRf (5 mL), and total alkalinity (60 mL). Nutrient concentrations of treatment bottles were analysed six times throughout the cruise using a QUAATRO39 (Seal Analytical) autoanalyzer.

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Hierarchy level: Dataset

Metadata

Metadata identifier: urn:uuid/493443ef-be06-40bf-b4af-7f6cd566c1a3

Language: English

Character encoding: UTF8

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IMAS Data Manager - Data Officer

Institute for Marine and Antarctic Studies

Private Bag 129

Hobart

Tasmania

7001

Australia

Institute for Marine and Antarctic Studies (IMAS) website >

Type of resource

Resource scope: Dataset

Name: IMAS Dataset level record

Metadata linkage: https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/493443ef-be06-40bf-b4af-7f6cd566c1a3
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Date info (Creation): 2024-06-24T00:00:00

Date info (Revision): 2024-08-30T14:32:51

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Title: ISO 19115-3:2018

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