IMAS Metadata Catalogue
Seasonality of phytoplankton growth limitation by iron and manganese in subantarctic waters
Phytoplankton indirectly influence climate through their role in the ocean biological carbon pump. In the Southern Ocean, the subantarctic zone represents an important carbon sink, yet variables limiting phytoplankton growth are not fully constrained. Using three shipboard bioassay experiments on three separate voyages, we evaluated the seasonality of iron (Fe) and manganese (Mn) co-limitation of subantarctic phytoplankton growth south of Tasmania, Australia. We observed a strong seasonal variation in a phytoplankton Fe limitation signal, with a summer experiment showing the greatest response to Fe additions. An autumn experiment suggested that other factors co-limited phytoplankton growth, likely low silicic acid concentrations. The phytoplankton responses to Mn additions were subtle and readily masked by the responses to Fe. Using flow cytometry, we observed that Mn may influence the growth of some small phytoplankton taxa in late summer/autumn, when they represent an important part of the phytoplankton community. In addition, Mn induced changes in the bulk photophysiology signal of the spring community. These results suggest that the importance of Mn may vary seasonally, and that its control on phytoplankton growth may be associated with specific taxa.
Simple
Identification info
- Date (Publication)
- 2023-06-06T00:00:00
Identifier
- Title
- Information and documentation - Digital object identifier system
- Citation identifier
- ISO 26324:2012
- Code
- 10.25959/BFF2-RC77
- Codespace
- doi.org
- Description
- Digital Object Identifier (DOI)
Principal investigator
Author
Author
Antarctic Climate and Ecosystems Cooperative Research Centre - Wuttig, Kathrin
University of Tasmania
Author
Author
Author
Author
- Credit
- We acknowledge the officers and crew of the RV Investigator (CSIRO Australian Marine National Facility; https://ror.org/01mae9353) for the deployment of all the instruments and the hydro-chemistry team who performed the macronutrient analyses onboard. This work was funded through the Antarctic Climate & Ecosystems Cooperative Centre (ACE CRC) and by the Australian Antarctic Program Partnership (AAPP; ASCI000002).
- Status
- Completed
Point of contact
Institute for Marine and Antarctic Studies - Latour, Pauline
University of Tasmania
Tasmania
Australia
- Topic category
-
- Biota
Extent
N
S
E
W
))
Temporal extent
- Time period
- 2018-09-12 2021-05-31
- Maintenance and update frequency
- Not planned
- Keywords (Theme)
-
- iron
- manganese
- co-limitation
- radioisotopes
- flow cytometry
- Global Change Master Directory Earth Science Keywords, Version 8.5
- AODN Discovery Parameter Vocabulary
-
- Concentration of ammonium {NH4} per unit volume of the water body
- Flow cytometry measurements
Resource constraints
- Use limitation
- Data, products and services from IMAS are provided "as is" without any warranty as to fitness for a particular purpose.
Resource constraints
- Linkage
-
https://licensebuttons.net/l/by/4.0/88x31.png
License Graphic
- Title
- Creative Commons Attribution 4.0 International License
- Alternate title
- CC-BY
- Edition
- 4.0
- Website
-
https://creativecommons.org/licenses/by/4.0/
License Text
- Other constraints
- Cite data as: Latour, P., Strzepek, R., Wuttig, K., van der Merwe, P., Bach, L., Eggins, S., Boyd, P., Ellwood, M., Pinfold, T., & Bowie, A. (2023). Seasonality of phytoplankton growth limitation by iron and manganese in subantarctic waters [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/BFF2-RC77
- Language
- English
- Character encoding
- UTF8
- Supplemental Information
- Seasonality of phytoplankton growth limitation by iron and manganese in subantarctic waters
Content Information
- Content type
- Physical measurement
Identifier
- Code
- Nitrate
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPOX
- Name
- Micromoles per litre
Identifier
- Code
- Silicate
- Name
- Silicate
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPOX
- Name
- Micromoles per litre
Identifier
- Code
- Phosphate
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPOX
- Name
- Micromoles per litre
Identifier
- Code
- Photochemical efficiency of Photosystem II
- Name
- Fv/Fm
- Name
- no unit
Identifier
- Code
- Functional absorption cross-section of Photosystem II
- Name
- nm-2 quanta-1
Identifier
- Code
- Dissolved iron concentrations
- Name
- dFe
- Name
- Nanomoles per litre
Identifier
- Code
- Dissolved manganese concentrations
- Name
- dMn
- Name
- Nanomoles per litres
- Name
- Ammonia
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPOX
- Name
- Micromoles per litre
Identifier
- Code
- Flow cytometry measurements
- Name
- no unit
Distribution Information
- Distribution format
-
- .xlsx, .fcs
- OnLine resource
- DATA ACCESS - browse all files available for download
- OnLine resource
- DATA ACCESS - download all files as. zip package
Resource lineage
- Statement
- We performed three field bioassays experiments onboard RV Investigator, in the subantarctic zone, to study iron (Fe) and manganese (Mn) co-limitation of phytoplankton growth. The first experiment (spring) was performed at Process Station 2 (45.44°S, 153.31°) during IN2018_V04 and the two other experiments (summer and autumn) were conducted at the Southern Ocean Time Series (SOTS) station (46.80°S, 141.884°E) during IN2019_V02 (autumn) and IN2020_V08 (summer). Unfiltered trace metal clean seawater was collected using a trace metal rosette and incubated with Fe and/or Mn additions in deck-board incubators. Neutral-density mesh bags were used to reproduce the light penetrating the surface ocean. Incubations bottles were sampled at the end of the experiment (day = 7) for macronutrient concentrations, measured through segmented flow analysis, photophysiology, measured using Light-induced Fluorescence Transients Fast Repetition Rate (LIFT-FRR) fluorometer and community composition using flow cytometry. Flow cytometry samples were fixed at sea and analysed back onshore using an Aurora Cytek flow cytometer (Cytek Biosciences). In addition, carbon and iron uptake rates were measured through radioisotopes additions: after 7 days of incubation, a portion of the incubated water was dispensed into smaller bottles and spiked with 14-carbon (NaH14CO3; specific activity 1.85 GBq mmol-1; PerkinElmer, USA) and 55-iron (55FeCl3 in 0.1 M Ultrapure HCl; specific activity 30 MBq mmol-1; PerkinElmer) solutions. Iron and carbon uptake rates were determined by measuring disintegrations per minute (DPM) on a liquid scintillation counter (PerkinElmer Tri-Carb 2910 TR) after incubation of the filters in Ultima Gold liquid scintillation cocktail at least 24h prior to analysis (PerkinElmer).
- Hierarchy level
- Dataset
Metadata
- Metadata identifier
- urn:uuid/2d9be445-e1da-4572-927b-b13bf7d1b601
- Language
- English
- Character encoding
- UTF8
Distributor
Type of resource
- Resource scope
- Dataset
- Name
- IMAS Dataset level record
- Metadata linkage
-
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/2d9be445-e1da-4572-927b-b13bf7d1b601
Point of truth URL of this metadata record
- Date info (Creation)
- 2023-05-05T00:00:00
- Date info (Revision)
- 2026-10-22T20:02:51
Metadata standard
- Title
- ISO 19115-3:2018
Overviews
Spatial extent
N
S
E
W
Provided by
Associated resources
Not available