Quantitative elemental imaging of octopus stylets using PIXE and the nuclear microprobe

By utilising targeted microprobe technology, the analysis of elements incorporated within the hard bio-mineralised structures of marine organisms has provided unique insights into the population biology of many species. As hard structures grow, elements from surrounding waters are incorporated effectively providing a natural ‘tag’ that is often unique to the animal’s particular location or habitat. The spatial distribution of elements within octopus stylets was investigated, using the nuclear microprobe, to assess their potential for determining dispersal and population structure in octopus populations. This was investigated in adult Octopus pallidus sourced from a commercial fishery in Tasmania.

Simple

Identification info

Date (Creation)
2011-10-26T15:36:00

Principal investigator

Tasmanian Aquaculture and Fisheries Institute (TAFI) - Doubleday, Zoe (PhD student)
Tasmanian Aquaculture and Fisheries Institute, University of Tasmania
Private Bag 49
Hobart
TAS
7001
Australia
ORCID ID >

Collaborator

Tasmanian Aquaculture and Fisheries Institute (TAFI) - Pecl, Gretta, Dr (PhD Supervisor)
Tasmanian Aquaculture and Fisheries Institute, University of Tasmania
Private Bag 49
Hobart
Tasmania
7001
Australia
ORCID ID >

Collaborator

Tasmanian Aquaculture and Fisheries Institute (TAFI) - Semmens, Jayson, Dr (PhD supervisor)
Tasmanian Aquaculture and Fisheries Institute, University of Tasmania
Private Bag 49
Hobart
Tasmania
7001
Australia
ORCID ID >

Credit
Winifred Violet Scott Estate Grant
Status
Completed

Principal investigator

School of Biological Sciences, The University of Adelaide - Doubleday, Zoe, Dr (Post-doctoral fellow)
The University of Adelaide
Adelaide
South Australia
5005
Australia
ORCID ID >

Collaborator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Pecl, Gretta, Dr
IMAS - Taroona
Private Bag 49
Hobart
TAS
7001
Australia
ORCID ID >

Topic category
  • Oceans

Extent

N
S
E
W


Temporal extent

Time period
2005-10-01T00:00:00 2006-01-31T00:00:00
Maintenance and update frequency
Not planned

Resource format

Title
Microsoft Excel
Date
Edition
2003
Global Change Master Directory Earth Science Keywords v5.3.8
  • Biosphere | Zoology | Mollusks
Keywords (Theme)
  • Nuclear microprobe
  • PIXE
  • Stylet
  • Elements
  • Spatial distribution
Keywords (Taxon)
  • Octopus pallidus
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
Keywords (Theme)
  • Element concentration

Resource constraints

Classification
Unclassified

Resource constraints

Use limitation
The data described in this record are the intellectual property of Zoe Doubleday.

Resource constraints

Linkage
http://i.creativecommons.org/l/by/2.5/au/88x31.png

License Graphic

Title
Creative Commons Attribution 2.5 Australia License


>

Website
http://creativecommons.org/licenses/by/2.5/au/

License Text

Other constraints
The citation in a list of references is: citation author name/s (year metadata published), metadata title. Citation author organisation/s. File identifier and Data accessed at (add http link).
Other constraints
Please contact Z. Doubleday for access to the data.

Associated resource

Title
Population structure of two commercial octopus species
Date (Creation)
2011-10-26T00:00:00
Language
English
Character encoding
UTF8
Environment description
Uni_file_Id: TAS_ZD_20070718_885
Supplemental Information
Doubleday, ZA, ‘An integrative approach to understanding the population structure and dispersal patterns of two commercial octopus species (Octopus maorum and Octopus pallidus)’ (2009). PhD thesis, University of Tasmania Doubleday, ZA and Belton, D and Pecl, GT and Semmens, JM, ‘Quantitative elemental imaging of octopus stylets using PIXE and the nuclear microprobe’, Nuclear Instruments and Methods in Physics Research B, 266 (1) pp. 67-72. ISSN 0168-583X (2008)

Content Information

Content type
Physical measurement
Name
Element concentration

Name
ppm

Distribution Information

Distribution format
  • Microsoft excel (xls)

Distributor

Principal investigator

School of Biological Sciences, The University of Adelaide - Doubleday, Zoe, Dr
ORCID ID >

Resource lineage

Statement
General composition analyses were performed on stylets collected from adult O. pallidus sourced from the commercial fishery in Tasmania in October 2005. Powder x-ray diffraction was conducted to determine mineral composition (Mineral Resources Tasmania, Australia). To determine total inorganic content 'loss-on-ignition' (combustion of organic material) was also conducted. Secondly, infrared spectroscopic analysis was conducted on the stylet with most of the outer sheath removed (Central Science Laboratory, University of Tasmania). For further details consult the published paper. Nuclear microprobe analysis Five stylets were sourced from adult O. pallidus also collected from the commercial fishery. Two 'juvenile' stylets were also sourced from five month old aquaria-reared O. pallidus in January 2006. For further details consult the published paper.
Hierarchy level
Dataset

Metadata

Metadata identifier
020ae2c0-44a6-11dc-8cd0-00188b4c0af8

Language
English
Character encoding
UTF8

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - IMAS Data Manager

Type of resource

Resource scope
Dataset
Metadata linkage
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/020ae2c0-44a6-11dc-8cd0-00188b4c0af8

Point of truth URL of this metadata record

Date info (Creation)
2015-09-03T15:08:45
Date info (Revision)
2015-09-03T15:08:45

Metadata standard

Title
ISO 19115-3:2018
 
 

Overviews

Spatial extent

N
S
E
W


Keywords

Element concentration Elements Nuclear microprobe PIXE Spatial distribution Stylet
Global Change Master Directory Earth Science Keywords v5.3.8
Biosphere | Zoology | Mollusks

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Not available