This record provides an overview of the NESP Marine and Coastal Hub bridging study - "Future-proofing restoration & thermal physiology of kelp". For specific data outputs from this project, please see child records associated with this metadata. -------------------- Kelp forests create complex habitats that support a diverse and productive community of marine life. They underpin coastal food-webs, fisheries, and a suite of other ecosystem services including nutrient and blue carbon cycling. Across much of the world, kelp forests are in decline and under threat from stressors including urbanisation, overgrazing, ocean warming, and marine heatwaves driven by climate change. Australia’s giant kelp (Macrocystis pyrifera) forests are listed as a Threatened Ecological Community under the Environment Protection and Biodiversity Conservation Act 1999. Habitat restoration is a potential tool for the conservation and management of giant kelp ecosystems. Given the direct impacts of climate change and ocean warming, there is growing recognition of the need for habitat restoration to be ‘future proofed’. For restoration to be effective, the cause of habitat decline must be understood and overcome. This is problematic when climate change is driving habitat loss since it cannot be reversed or ameliorated prior to restoration. A previous NESP project led by this team (NESP Marine Biodiversity Hub Project E7) identified warm-tolerant strains of giant kelp from remnant patches in eastern Tasmania, where the species has experienced precipitous declines due to ocean-warming. These strains have high potential to assist with ‘future-proofing’ kelp forest restoration, however it is still unclear what the physiological mechanisms are that provide their improved thermal tolerance. It is also unknown whether cross-breeding the identified warm-tolerant giant kelp strains will affect and potentially improve their thermal tolerance capacity. This project explored the physiology of kelp thermal performance, specifically the mechanisms potentially responsible for the warm water tolerance identified in particular giant kelp strains. It confirmed the improved ability of the warm-tolerant strains to develop at stressful warm temperatures relative to normal giant kelp, and demonstrated for the first time that their improved thermal performance may extend to the development and fertilisation. The outcomes progress toward the identification of populations of Australian kelp that may be resilient to (or especially threatened by) ocean warming and climate change. Outputs • Ecophysiological measurements from laboratory experiments of warm-tolerant vs average giant kelp genotypes [dataset] • Final Project Report including a short summary of recommendations for policy makers of key findings [written]

The data is quantitative abundance of fish, megafaunal invertebrates and percent algal cover derived from underwater visual census methods involving transect counts at rocky reef sites around Tasmania. This dataset allows examination of changes in Tasmanian shallow reef faunal and floral communities over a decadal scale, with initial surveys conducted in 1992-1995, and again in 2006-2007. There are plans for ongoing surveys. An additional component was added in the latter study - a boat ramp study looking at the proximity of boat ramps and their effects of fishing. We analysed underwater visual census data on fishes and macroinvertebrates (abalone and rock lobsters) at 133 shallow rocky reef sites around Tasmania that ranged from 0.6 - 131 km from the nearest boat ramp. These sites were not all the same as those used for the comparison of 1994 and 2006 reef communities. The subset of 133 sites examined in this component consisted of only those sites that were characterized by the two major algal (kelp) types (laminarian or fucoid dominated). Sites with atypical algal assemblages were omitted from the 196 sites surveyed in 2006. This study aimed to examine reef community data for changes at the community level, changes in species richness and introduced species populations, and changes that may have resulted from ocean warming and fishing. The methods are described in detail in Edgar and Barrett (1997). Primarily the data are derived from transects at 5 m depth and/or 10 m depth at each site surveyed. The underwater visual census (UVC) methodology used to survey rocky reef communities was designed to maximise detection of (i) changes in population numbers and size-structure (ii) cascading ecosystem effects associated with disturbances such as fishing, (iii) long term change and variability in reef assemblages.

The main purpose of the study was to gain a greater understanding of the chemical and biological characteristics of sites in the D'Entrecasteaux Channel and offshore of the Derwent Estuary between North West Bay and Betsey Island. Four transects were completed on the 5th and 6th of April 2022 to achieve this. A SBE 55 ECO Water Sampler Conductivity, Temperature and Depth (CTD) Rosette was used to collect depth, temperature, conductivity, salinity, density, dissolved oxygen, pH, turbidity, fluorescence and pressure data. Water samples collected at three depths using Niskin bottles were used to obtain discrete measurements of oxygen, pH, alkalinity and phosphorous at sea, and discrete measurements of chlorophyll-a, ammonia, NOx, nitrite, nitrate, phosphate and silicate in a laboratory setting. A plankton tow net was used to roughly measure the abundance of plankton at each site and a Secchi disk was used to discern the Secchi depth.

The aim of this trip was to investigate the salt wedge of the Derwent estuary, looking at the different properties along and across the estuary. This was achieved by examining; temperature, salinity, chlorophyll-a concentration, nutrients (including nitrate, ammonia, phosphate and silicate), pressure, turbidity, fluorescence, dissolved oxygen and pH. Sampling took place in the morning, in sunny, relatively calm conditions with the low tide on the sampling day at approximately 11:04 am. Two transects were used, with three different locations tested along the estuary and four tested across, at the approximate locations: (-42.94014, 147.38385), (-42.96321, 147.57525), (-42.98388, 147.37083), (-42.9795, 147.336), (-42.9744, 147.3585), (-42.97594, 147.36394) and (-42.97616, 147.38914). Sampling consisted of CTD sensor measurements, a plankton net tow, Secchi disk measurements, and a collection of water samples for analysis.