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This database contains sampling effort, catch records, biological data, and water quality data for sampling and catches of elasmobranchs in northern Australian rivers, estuaries and coasts undertaken under the National Environmental Research Program (NERP) Marine Biodiversity Hub Project 2.4 'Supporting Management of Listed and Rare Species'. and the National Environmental Science Program (NESP) Marine Biodiversity Hub Project A1 'Northern Australian Hotspots for the Recovery of Threatened Euryhaline Elasmobranchs'. Surveys using gillnets and rod-and-line were undertaken in the Top End region of the Northern Territory and the Kimberley region of Western Australia. Selected animals were tagged for movement ecology, habitat use and mortality estimates (acoustic telemetry), and tissue samples were collected from all fish for molecular analyses (population genetics and close-kin mark-recapture).