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Environmental DNA data from the RV Investigator South-East Australian Marine Ecosystem Survey (SEA-MES) voyages IN2023_V05 and IN2024_V03 (NESP MaC 4.23)

This project generated a set of high-quality eDNA-based biodiversity datasets from samples collected at broadly distributed offshore sites across Southeast Australia. The data consists of processed data from eDNA metabarcoding assays (i.e. tables with sampling information, sequence read counts and taxonomy derived from the sequences). Raw sequence data (over 180 million sequences) is available from NCBI ( https://www.ncbi.nlm.nih.gov/) under BioProject PRJNA1263580: Environmental DNA measuring offshore marine biodiversity in southeast Australia ( https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1263580/).


The eDNA biodiversity information focussed on fish but includes sequence data from the full tree of life (from bacteria to vertebrates). Part of the purpose of data collection was to assess key technical aspects of eDNA data collection to refine sampling methods and provide guidance for best practices in future offshore eDNA research

Simple

Identification info

Date (Publication)
2025-10-20T00:00:00

Identifier

Title
Information and documentation - Digital object identifier system
Citation identifier
ISO 26324:2012

Code
10.25959/AY48-9W15
Codespace
doi.org
Description
Digital Object Identifier (DOI)

Principal investigator

CSIRO Environment - Deagle, Bruce (Project Leader)
ROR ID >

ORCID >

Collaborator

CSIRO Environment - Bodrossy, Levente
ROR ID >

ORCID >

Collaborator

CSIRO Environment - Bessey, Cindy
ROR ID >

ORCID >

Collaborator

CSIRO Environment - Jayasinghe, Sahan
ROR ID >

ORCID >

Collaborator

CSIRO Environment - West, Katrina
ROR ID >

ORCID >

Credit
The data collections described in this record are funded by the Australian Government Department of Climate Change, Energy, the Environment and Water (DCCEEW) through the NESP Marine and Coastal Hub. In addition to NESP (DCCEEW) funding, this project is matched by an equivalent amount of in-kind support and co-investment from project partners and collaborators.
Credit
We also acknowledge the use of the CSIRO Marine National Facility (https://ror.org/01mae9353) and grant of sea time on RV Investigator in undertaking this research. We thank the entire South-East Australian Marine Ecosystem Survey (SEA-MES) team, in particular Rich Little (CSIRO), Chief Scientist on the SEA-MES voyages, whose support was key to incorporating eDNA into the broader research program.
Status
Completed

Point of contact

CSIRO Environment - Deagle, Bruce
Tasmania
Australia
ROR ID >

ORCID >

Topic category
  • Biota
  • Environment

Extent



Temporal extent

Time period
2023-07-01 2024-06-01
Maintenance and update frequency
Not planned
Keywords (Project)
  • National Environmental Science Program (NESP) Marine and Coastal Hub
Keywords (Theme)
  • Environmental DNA
  • Southeast Australia Marine Ecosystem Survey
  • Biomonitoring
  • Marine Biodiversity Survey
  • RV Investigator
GCMD Earth Science keywords
  • MARINE ECOSYSTEMS
  • MARINE ENVIRONMENT MONITORING
AODN Geographic Extents Vocabulary
  • Coastal Waters (Australia) | Tasmania Coast East and Southeast, TAS
  • Regional Seas | Tasman Sea
  • Marine Planning Regions (Australia) | South-east
  • Marine Features (Australia) | Bass Strait, TAS/VIC
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
  • Environmental Monitoring
  • Marine and Estuarine Ecology (incl. Marine Ichthyology)
  • Population, Ecological and Evolutionary Genetics

Resource constraints

Other constraints
This dataset is hosted by the University of Tasmania, on behalf of CSIRO and NESP Marine and Coastal Hub Project 4.23.

Resource constraints

Classification
Unclassified

Resource constraints

Use limitation
Data was sourced from the NESP Marine and Coastal Hub – the Marine and Coastal Hub is supported through funding from the Australian Government’s National Environmental Science Program (NESP), administered by the Department of Climate Change, Energy, the Environment and Water.

Resource constraints

Linkage
https://licensebuttons.net/l/by/4.0/88x31.png

License Graphic

Title
Creative Commons Attribution 4.0 International License
Alternate title
CC-BY
Edition
4.0


>

Website
https://creativecommons.org/licenses/by/4.0/

License Text

Other constraints
Cite data as: Deagle, B., Bodrossy, L., Bessey, C., Jayasinghe, S., & West, K. (2025). Environmental DNA data from the RV Investigator South-East Australian Marine Ecosystem Survey (SEA-MES) voyages IN2023_V05 and IN2024_V03 [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/AY48-9W15

Associated resource

Title
NESP MaC Project 4.23 - Environmental DNA for measuring offshore marine biodiversity: what can DNA in water collected from the RV Investigator tell us?, 2024-2025 (CSIRO)

Identifier

Code
10.71676/d0dfa7a2
Codespace
raid.org
Description
Project RAiD
Association Type
Dependency
Initiative Type
Project

Associated resource

Association Type
Cross reference
Title
CSIRO Data Access Portal copy of dataset

Identifier

Code
10.25919/ngqx-3557
Codespace
doi.org
Language
English
Character encoding
UTF8

Distribution Information

OnLine resource
DATA ACCESS - browse and download available files

OnLine resource
Final Project Report

Deagle BE, Jayasinghe SA, Bessey C, Bodrossy L, West, KM (2025) Environmental DNA for measuring offshore marine biodiversity: what can DNA in water collected from the RV Investigator tell us? Report to the National Environmental Science Program. CSIRO, Hobart, Australia.

Resource lineage

Statement
eDNA samples were collected from the CSIRO research vessel (RV) Investigator as part of the first two SEA-MES voyages: IN2023_V05 (July 2023) and IN2024_V03 (May 2024). Sampling occurred at sites along the edge of the continental shelf (~80 to 500 m) stretching from the east coast of Tasmania to Batemans Bay (NSW). The eDNA samples primarily came from water collected from Niskin bottles on a CTD rosette. An eDNA sampler was also deployed on the deep tow camera system to collect eDNA when this device was towed along transects near the bottom. During the two voyages, water was collected from over 100 CTD deployments, with most samples collected from near the surface and bottom. eDNA metabarcoding was used to obtain sequence information from the samples. This project used several different eDNA markers (also referred to as assays) allowing detection of a broad range of marine species. The total sequencing of eDNA produced over 180 million sequences. Markers included Fish (mtDNA 16S), Metazoan (mtDNA COI Leray) and Eukaryote (V4 18S) and a panel of 12 different markers were generated (targets include bacteria, crustaceans, fish, etc.).
Hierarchy level
Dataset

Metadata

Metadata identifier
urn:uuid/98fb2a49-4082-4648-a618-19270f554e88

Language
English
Character encoding
UTF8

Point of contact

Institute for Marine and Antarctic Studies - Emma Flukes (NESP Marine and Coastal Hub Data Manager (Southern node))
Parent metadata
  • NESP MaC Project 4.23 - Environmental DNA for measuring offshore marine biodiversity: what can DNA in water collected from the RV Investigator tell us?, 2024-2025 (CSIRO)

Type of resource

Resource scope
Dataset
Metadata linkage
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/98fb2a49-4082-4648-a618-19270f554e88

Point of truth URL of this metadata record

Date info (Creation)
2025-10-20
Date info (Revision)
2026-05-19T17:35:09

Metadata standard

Title
ISO 19115-3:2018
 
 

Overviews

Spatial extent

Keywords

Biomonitoring Environmental DNA Marine Biodiversity Survey RV Investigator Southeast Australia Marine Ecosystem Survey
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
Environmental Monitoring Marine and Estuarine Ecology (incl. Marine Ichthyology) Population, Ecological and Evolutionary Genetics
GCMD Earth Science keywords
MARINE ECOSYSTEMS MARINE ENVIRONMENT MONITORING

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Associated resources

Not available


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