Environmental DNA data from the RV Investigator South-East Australian Marine Ecosystem Survey (SEA-MES) voyages IN2023_V05 and IN2024_V03 (NESP MaC 4.23)
This project generated a set of high-quality eDNA-based biodiversity datasets from samples collected at broadly distributed offshore sites across Southeast Australia. The data consists of processed data from eDNA metabarcoding assays (i.e. tables with sampling information, sequence read counts and taxonomy derived from the sequences). Raw sequence data (over 180 million sequences) is available from NCBI ( https://www.ncbi.nlm.nih.gov/) under BioProject PRJNA1263580: Environmental DNA measuring offshore marine biodiversity in southeast Australia ( https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1263580/).
The eDNA biodiversity information focussed on fish but includes sequence data from the full tree of life (from bacteria to vertebrates). Part of the purpose of data collection was to assess key technical aspects of eDNA data collection to refine sampling methods and provide guidance for best practices in future offshore eDNA research
Simple
Identification info
- Date (Publication)
- 2025-10-20T00:00:00
Identifier
- Title
- Information and documentation - Digital object identifier system
- Citation identifier
- ISO 26324:2012
- Code
- 10.25959/AY48-9W15
- Codespace
- doi.org
- Description
- Digital Object Identifier (DOI)
Principal investigator
- Credit
- The data collections described in this record are funded by the Australian Government Department of Climate Change, Energy, the Environment and Water (DCCEEW) through the NESP Marine and Coastal Hub. In addition to NESP (DCCEEW) funding, this project is matched by an equivalent amount of in-kind support and co-investment from project partners and collaborators.
- Credit
- We also acknowledge the use of the CSIRO Marine National Facility (https://ror.org/01mae9353) and grant of sea time on RV Investigator in undertaking this research. We thank the entire South-East Australian Marine Ecosystem Survey (SEA-MES) team, in particular Rich Little (CSIRO), Chief Scientist on the SEA-MES voyages, whose support was key to incorporating eDNA into the broader research program.
- Status
- Completed
Point of contact
- Topic category
-
- Biota
- Environment
Extent
Temporal extent
- Time period
- 2023-07-01 2024-06-01
- Maintenance and update frequency
- Not planned
- Keywords (Project)
-
- National Environmental Science Program (NESP) Marine and Coastal Hub
- Keywords (Theme)
-
- Environmental DNA
- Southeast Australia Marine Ecosystem Survey
- Biomonitoring
- Marine Biodiversity Survey
- RV Investigator
Resource constraints
- Other constraints
- This dataset is hosted by the University of Tasmania, on behalf of CSIRO and NESP Marine and Coastal Hub Project 4.23.
Resource constraints
- Classification
- Unclassified
Resource constraints
- Use limitation
- Data was sourced from the NESP Marine and Coastal Hub – the Marine and Coastal Hub is supported through funding from the Australian Government’s National Environmental Science Program (NESP), administered by the Department of Climate Change, Energy, the Environment and Water.
Resource constraints
- Linkage
-
https://licensebuttons.net/l/by/4.0/88x31.png
License Graphic
- Title
- Creative Commons Attribution 4.0 International License
- Alternate title
- CC-BY
- Edition
- 4.0
- Website
-
https://creativecommons.org/licenses/by/4.0/
License Text
- Other constraints
- Cite data as: Deagle, B., Bodrossy, L., Bessey, C., Jayasinghe, S., & West, K. (2025). Environmental DNA data from the RV Investigator South-East Australian Marine Ecosystem Survey (SEA-MES) voyages IN2023_V05 and IN2024_V03 [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/AY48-9W15
Associated resource
- Title
- NESP MaC Project 4.23 - Environmental DNA for measuring offshore marine biodiversity: what can DNA in water collected from the RV Investigator tell us?, 2024-2025 (CSIRO)
Identifier
- Association Type
- Dependency
- Initiative Type
- Project
Associated resource
- Association Type
- Cross reference
- Title
- CSIRO Data Access Portal copy of dataset
Identifier
- Code
- 10.25919/ngqx-3557
- Codespace
- doi.org
- Language
- English
- Character encoding
- UTF8
Distribution Information
- OnLine resource
- DATA ACCESS - browse and download available files
- OnLine resource
-
Final Project Report
Deagle BE, Jayasinghe SA, Bessey C, Bodrossy L, West, KM (2025) Environmental DNA for measuring offshore marine biodiversity: what can DNA in water collected from the RV Investigator tell us? Report to the National Environmental Science Program. CSIRO, Hobart, Australia.
Resource lineage
- Statement
- eDNA samples were collected from the CSIRO research vessel (RV) Investigator as part of the first two SEA-MES voyages: IN2023_V05 (July 2023) and IN2024_V03 (May 2024). Sampling occurred at sites along the edge of the continental shelf (~80 to 500 m) stretching from the east coast of Tasmania to Batemans Bay (NSW). The eDNA samples primarily came from water collected from Niskin bottles on a CTD rosette. An eDNA sampler was also deployed on the deep tow camera system to collect eDNA when this device was towed along transects near the bottom. During the two voyages, water was collected from over 100 CTD deployments, with most samples collected from near the surface and bottom. eDNA metabarcoding was used to obtain sequence information from the samples. This project used several different eDNA markers (also referred to as assays) allowing detection of a broad range of marine species. The total sequencing of eDNA produced over 180 million sequences. Markers included Fish (mtDNA 16S), Metazoan (mtDNA COI Leray) and Eukaryote (V4 18S) and a panel of 12 different markers were generated (targets include bacteria, crustaceans, fish, etc.).
- Hierarchy level
- Dataset
Metadata
- Metadata identifier
- urn:uuid/98fb2a49-4082-4648-a618-19270f554e88
- Language
- English
- Character encoding
- UTF8
Point of contact
Type of resource
- Resource scope
- Dataset
- Metadata linkage
-
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/98fb2a49-4082-4648-a618-19270f554e88
Point of truth URL of this metadata record
- Date info (Creation)
- 2025-10-20
- Date info (Revision)
- 2026-05-19T17:35:09
Metadata standard
- Title
- ISO 19115-3:2018
Overviews
Spatial extent
Provided by
IMAS Metadata Catalogue