Phenotypic plasticity in habitat-forming seaweeds subject to latitudinal transplant
The phenotypic plasticity of habitat-forming seaweeds was investigated with a transplant experiment in which juvenile Ecklonia radiata and Phyllospora comosa were transplanted from NSW (warm conditions) to Tasmania (cool conditions) and monitored for four months. We used multiple performance indicators (growth, photosynthetic characteristics, pigment content, chemical composition, stable isotopes, nucleic acids) to assess the ecophysiology of seaweeds before and following transplantation between February 2012 and June 2012.
Simple
Identification info
- Date (Creation)
- 2016-02-22
Identifier
- Title
- Information and documentation - Digital object identifier system
- Citation identifier
-
ISO 26324:2012
- Codespace
- doi.org
- Description
- Digital Object Identifier (DOI)
Principal investigator
Collaborator
Collaborator
- Credit
- Australian Research Council (ARC) grant DP1096573
- Status
- Completed
Point of contact
- Temporal resolution
-
P0Y1M0DT0H0M0S
- Topic category
-
- Biota
Extent
Temporal extent
- Time period
- 2012-02-25 2012-06-30
Vertical element
- Minimum value
- 5
- Maximum value
- 10
- Identifier
- EPSG:5715
- Name
- MSL depth
- Maintenance and update frequency
- Not planned
Resource format
- Title
- CSV
- Date
- Edition
- n/a
- Keywords (Theme)
-
- phycology
- kelp
- PAM fluorometry
- ecophysiology
- RNA:DNA
- transplantation
- ecosystem engineer
- phenotypic plasticity
- in situ measurements
- Keywords (Taxon)
-
- Ecklonia radiata
- Phyllospora comosa
- NASA/GCMD Keywords, Version 8.5
-
- EARTH SCIENCE | BIOSPHERE | VEGETATION | CHLOROPHYLL
- EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS)
- EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS) | BROWN ALGAE
- EARTH SCIENCE | BIOSPHERE | VEGETATION | PIGMENTS
- EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS
- EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | REEF
- EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | BENTHIC
- Keywords (Discipline)
-
- Temperate Reef
- AODN Instrument Vocabulary
Resource constraints
- Classification
- Unclassified
Resource constraints
- Linkage
-
http://i.creativecommons.org/l/by/4.0/88x31.png
License Graphic
- Title
- Creative Commons Attribution 4.0 International License
- Website
-
http://creativecommons.org/licenses/by/4.0/
License Text
- Other constraints
- Cite data as: Flukes, E., Johnson, C., & Wright, J. (2016). Phenotypic plasticity in habitat-forming seaweeds subject to latitudinal transplant [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.4226/77/572171CF5940F
- Other constraints
- The data described in this record are the intellectual property of the University of Tasmania through the Institute for Marine and Antarctic Studies.
- Language
- English
- Character encoding
- UTF8
Content Information
- Content type
- Physical measurement
- Description
- measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
- Name
-
Relative electron transport rate
- Name
-
rETRmax
- Name
- no units (relative units)
- Description
- measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
- Name
-
Alpha
- Name
-
Alpha
- Name
- nil
- Description
- measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
- Name
-
Minimum saturating irradiance
- Name
-
Ek
- Name
- µmol photons m-2 s-1
- Description
- measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
- Name
-
Maximum quantum yield (dark-acclimated sample) - also Fv/Fm
- Name
-
Fv.Fm
- Name
- nil (ratio)
- Name
-
Tissue concentration of fucoxanthin
- Name
-
Fucoxanthin
- Name
- mg g-1 wet weight
- Name
-
Tissue concentration of chlorophyll a
- Name
-
ChlA
- Name
- mg g-1 wet weight
- Name
-
Tissue concentration of chlorophyll c
- Name
-
ChlC
- Name
- mg g-1 wet weight
- Description
- Both RNA and DNA concentrations were extracted from a single sample of tissue
- Name
-
Ratio of tissue RNA to DNA concentration
- Name
-
RNA.DNA
- Name
- nil (ratio)
- Description
- measured using extraction method developed specifically for this data
- Name
-
Tissue concentration of RNA
- Name
-
RNA
- Name
- µg g wet weight-1
- Description
- measured using extraction method developed specifically for this data
- Name
-
Tissue concentration of DNA
- Name
-
DNA
- Name
- µg g wet weight-1
- Name
-
Tissue percentage carbon
- Name
-
per_C
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPCT
- Name
- Percent
- Name
-
Tissue percentage nitrogen
- Name
-
per_N
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPCT
- Name
- Percent
- Description
- measured using mass spectrometry
- Name
-
Ratio of tissue carbon to nitrogen
- Name
-
C.N
- Name
- nil (ratio)
- Description
- Pee Dee Belemnite as standard
- Name
-
δ13C
- Name
-
d13C
- Name
- ‰
- Description
- Air as standard
- Name
-
δ15N
- Name
-
d15N
- Name
- ‰
- Description
- measured as length x width of blades/lamina
- Name
-
Blade area
- Name
-
Blade_area
- Name
- mm^2
Distribution Information
- Distribution format
-
-
CSV (.csv)
-
CSV (.csv)
- OnLine resource
-
DATA - Ecophysiological measurements prior to, and 4 months following transplantation (.csv) [direct download]
- OnLine resource
-
DATA - Raw PAM fluorometry RLC outputs at monthly intervals (zipped .csv) [direct download]
- OnLine resource
-
ASSOCIATED PUBLICATION - Ecophysiology of habitat-forming seaweeds in a changing environment [PhD thesis]
See Chapter 2
Resource lineage
- Statement
- Juvenile Ecklonia radiata and Phyllospora comosa were transplanted from NSW-Tas and Tas-Tas. Seaweeds were first collected and kept overnight in the laboratory where they were attached to lengths of 6 mm diameter polypropylene rope by inserting holdfasts into the weave of the rope. Phyllospora were outplanted at a depth of ~5m and Ecklonia at ~10m in Fortescue Bay, Tasmania on 28th/29th February 2012. Measurements of growth and PAM fluorometry were made monthly, while other destructive ecophysiological measurements were made in situ at the time of collection, and 123 days following transplantation. The following parameters were measured: growth, PAM fluorometry photosynthetic characteristics, pigment content (chlorophyll a, c, fucoxanthin), tissue chemistry (δ13C, δ15N, % C, % N, C:N ratios), and nucleic acid characteristics (absolute RNA and DNA, RNA:DNA ratios). Terminal measurements were made on 30th June 2012. Pigments were measured using spectrophotometry and HPLC. Tissue chemistry was measured using mass spectrometry. Nucleic acids were measured using a novel technique described in Flukes et al. 2016. See Flukes et al. 2016 for a full description of all methodologies. NOTE: in data file "otherparamdata.csv", "start" sampling refers to in situ samples, "end" sampling refers to terminal measurements at t = 123 days. In data files "growthdata.csv" and contents of "raw_PAM_RLC_output", sample times refer to time since transplantation (days).
- Hierarchy level
- Dataset
- Hierarchy level
- Dataset
Platform
Identifier
- Code
- diver
Instrument
Identifier
- Code
- fluorometers
- Hierarchy level
- Dataset
Instrument
Identifier
- Hierarchy level
- Dataset
Instrument
Identifier
- Code
- spectrophotometers
- Hierarchy level
- Dataset
Instrument
Identifier
Metadata
- Metadata identifier
-
urn:uuid/6cbf0c73-1ade-4c1d-a83f-555118cf15ea
- Language
- English
- Character encoding
- UTF8
Author
Point of contact
Type of resource
- Resource scope
- Dataset
- Metadata linkage
-
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/6cbf0c73-1ade-4c1d-a83f-555118cf15ea
Point of truth URL of this metadata record
- Date info (Creation)
- 2020-08-14T12:02:23
- Date info (Revision)
- 2025-01-19T20:30:53
Metadata standard
- Title
- ISO 19115-3:2018