Carbon and nitrogen isotope data for J. edwardsii lobsters from eight sites in SE Australia.

These files contain the data recorded from a mesocosm experiment conducted in Bergen, Norway 2022 which assessed the effect of simualted mineral-based (silicate or calcium) ocean alkalinity enhancement (OAE) on diatom silicification. Ten mesocosms were used in total, divided into two groups either the silicate- or calcium based group and alkalinity was increased by either 0, 150, 300, 450 or 600 µmol L-1 above natrually occuring levels. The PDMPO-fluorescence (an appropriate proxy for silicification) of diatoms was recorded on eight seperate days during the experiment. Accompanying data includes measured; macronutrients (nitrate, nitrite, phophate, silicate), total alkalinity, biogenic silica in the water column and sediment trap.

We compare the formulation and emergent dynamics of 11 CMIP6 IPCC marine biogeochemical models. We find that the largest source of uncertainty across model simulations of marine carbon cycling is grazing pressure (i.e. the phytoplankton specific loss rate to grazing). Variability in grazing pressure is driven by large differences in zooplankton specific grazing rates, which are not sufficiently compensated for by offsetting differences in zooplankton specific mortality rates. Models instead must tune the turnover rate of the phytoplankton population to balance large differences in top-down grazing pressure and constrain net primary production. We then run a controlled sensitivity experiment in a global, coupled ocean-biogeochemistry model to test the sensitivity of marine carbon cycling to this uncertainty and find that even when tuned to identical net primary production, export and secondary production remain extremely sensitive to grazing, likely biasing predictions of future climate states and food security.

Diel partitioning of animals within ecological communities is widely acknowledged, yet rarely quantified. Investigation of most ecological patterns and processes involves convenient daylight sampling, with little consideration of the contributions of nocturnal taxa, particularly in marine environments. Here we assess diel partitioning of reef faunal assemblages at a continental scale utilizing paired day and night visual census across 54 shallow tropical and temperate reefs around Australia. Day/night differences were most pronounced in the tropics, with fishes and invertebrates displaying distinct and opposing diel occupancy on coral reefs. Tropical reefs in daytime were occupied primarily by fishes not observed at night (64% of all species sighted across day and night, and 71% of all individuals). By night, substantial emergence of invertebrates not otherwise detected during sunlit hours occurred (56% of all species, and 45% of individuals). Nocturnal emergence of tropical invertebrates corresponded with significant declines in the richness and biomass of predatory and herbivorous diurnal fishes. In contrast, relatively small diel changes in fishes active on temperate reefs corresponded to limited nocturnal emergence of temperate invertebrates. This reduced partitioning may, at least in part, be a result of strong top-down pressures from fishes on invertebrate communities, either by predation or competitive interference. For shallow reefs, the diel cycle triggers distinct emergence and retreat of faunal assemblages and associated trophic patterns and processes, which otherwise go unnoticed during hours of regular scientific monitoring. Improved understanding of reef ecology, and management of reef ecosystems, requires greater consideration of nocturnal interactions. Without explicit sampling of nocturnal patterns and processes, we may be missing up to half of the story when assessing ecological interactions.

Ocean alkalinity enhancement (OAE) is an emerging carbon dioxide removal (CDR) strategy that leverages the natural processes of weathering and acid neutralisation to durably store atmospheric CO2 in seawater. OAE can be achieved with a variety of methods, all of which have different environmental implications. One widely considered method utilizes electrochemistry to remove strong acid from seawater, leaving sodium hydroxide (NaOH) behind. This study evaluates the impacts of OAE via NaOH (NaOH-OAE) on a coastal plankton bloom, with particular focus on how macronutrient regeneration in the aftermath of the bloom responds to the perturbation. To investigate this, we enclosed a natural coastal phytoplankton community, including coccolithophores, in nine microcosms. The microcosms were divided into three groups: control, unequilibrated (512.1 ± 2.5 µmol kg-1 alkalinity increase) and equilibrated (499.3 ±5.65 µmol kg-1 alkalinity increase). Light was provided for 11 days to stimulate a bloom (light phase) and lights were turned off thereafter to investigate alkalinity and nutrient changes for 21 days (dark phase). We found no detectable effect of equilibrated NaOH-OAE on phytoplankton community and bacteria abundances determined with flow cytometry but observed a small yet detectable restructuring of phytoplankton communities under unequilibrated conditions. NaOH-OAE had no significant effect on alkalinity, NOx- and phosphate regeneration, but increased silicate regeneration by 64% over 21 days under darkness in the unequilibrated treatments where seawater pH was highest (8.65 relative to 7.92 in the control). Additional dissolution experiments with two diatom species supported this outcome on silicate regeneration for one of the two species, thereby suggesting that the effect is species specific. Our results point towards the potential of NaOH-OAE to influence regeneration of silicate in the surface ocean and thus the growth of diatoms, at least under the very extreme NaOH-OAE conditions simulated here.

In this dataset, we compared growth of Fraglariopsis cylindrus and Phyaeocystis antarctica collected from coastal and open ocean water at 3 °C, 5 °C and 7 °C, with and without additions of 5 nM dFe(II).

Invasive mammal eradications are commonplace in island conservation. However, post-eradication monitoring beyond the confirmation of target species removal is rarer. Seabirds are ecosystem engineers on islands and are negatively affected by invasive mammals. Following an invasive mammal eradication, the recovery of seabird populations can be necessary for wider ecosystem recovery. Seabirds fertilise islands with isotopically heavy nitrogen, which means nitrogen stable isotope analysis (δ15N) could provide a useful means for assessing corresponding change in ecosystem function. We quantified decadal changes in δ15N on eight temperate New Zealand islands subject in pairs to distinct mammal invasion and seabird restoration histories: invaded, never-invaded, invader-eradicated and undergoing active seabird restoration. First, we investigated long-term changes in δ15N values on individual islands. Second, we used a space for time analysis to determine if δ15N levels on islands from which invaders had been removed eventually recovered to values typical of never-invaded islands. On each island soil, plants (Coprosma repens, C. robust and Myrsine australis) and spiders (Porrhothelidae) were sampled in 2006/07 and 2022 allowing δ15N change on individual islands over 16 years to be assessed. Combined, the samples from invader-eradicated islands provided a 7 – 32 year post-eradication dataset. Change in δ15N was only detected on one island across the study period, following the unexpected recolonisation of seabirds to an invaded island. Invader-eradicated islands generally had higher δ15N values than invaded islands however, they were still lower than never-invaded islands and there was no trend in δ15N with time since eradication. This, and the measurable increase in δ15N following seabird recolonisation on one island, may suggest that δ15N change occurs rapidly following invader-eradication, but then slows, with δ15N values staying relatively constant in the time period studied here. Isotope and seabird population studies need to be coupled to ascertain if plateauing in δ15N reflects a slowing of seabird population growth and subsequent basal nutrient input, or if the baseline nutrients are entering the ecosystem but then not propagating up the food web.

A compilation of existing literature on the characteristics of Southern Ocean diatom species.