This project generated a set of high-quality eDNA-based biodiversity datasets from samples collected at broadly distributed offshore sites across Southeast Australia. The data consists of processed data from eDNA metabarcoding assays (i.e. tables with sampling information, sequence read counts and taxonomy derived from the sequences). Raw sequence data (over 180 million sequences) is available from NCBI (https://www.ncbi.nlm.nih.gov/) under BioProject PRJNA1263580: Environmental DNA measuring offshore marine biodiversity in southeast Australia (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1263580/). The eDNA biodiversity information focussed on fish but includes sequence data from the full tree of life (from bacteria to vertebrates). Part of the purpose of data collection was to assess key technical aspects of eDNA data collection to refine sampling methods and provide guidance for best practices in future offshore eDNA research

This record provides an overview of the NESP Marine and Coastal Hub Research Plan 2024 project "Environmental DNA for measuring offshore marine biodiversity: what can DNA in water collected from the RV Investigator tell us?". For specific data outputs from this project, please see child records associated with this metadata. -------------------- Environmental DNA (eDNA) in seawater provides a non-extractive way to characterise marine biodiversity from genetic material shed by organisms, from microbes and plankton to invertebrates and fishes. It is an emerging tool for marine ecosystem monitoring, particularly in offshore areas where conventional sampling can be difficult, costly or environmentally disruptive. This project collected and analysed more than 500 eDNA samples from 91 sites during the South-east Australian Marine Ecosystem Survey (SEA-MES) on RV Investigator. Samples were collected along the continental shelf between Tasmania and southern New South Wales, including sites within the South-east Marine Parks Network, providing the first comprehensive eDNA baseline for this region. The project tested active eDNA sampling from CTD water samples and passive eDNA sampling from a deep-towed camera system. DNA metabarcoding and Tree of Life metabarcoding were used to identify biodiversity patterns across the water column and near the seafloor. Results were compared with conventional survey methods, including fish trawls, plankton sampling and deep-towed camera deployments, to assess how eDNA complements existing monitoring approaches. The dataset revealed clear biodiversity patterns by depth, latitude, and water-column position, including higher fish diversity near the seafloor than at the surface. Comparisons with trawl and plankton data showed that eDNA provided complementary biodiversity information, supporting its use alongside existing survey methods and highlighting its value as part of integrated monitoring programs. The outputs provide a new biodiversity baseline for south-eastern Australian waters, and practical guidance on future application of eDNA techniques for offshore monitoring of marine parks and fisheries. Outputs • eDNA sequences with associated collection metadata (x2 voyages x50 sites) [dataset] • Final project report [written]

The Aqua and Orbview satellites carry a MODIS and SeaWIFS sensors (respectively) that observes sunlight reflected from within the ocean surface layer at multiple wavelengths. These multi-spectral measurements are used to infer the concentration of chlorophyll-a (Chl-a), most typically due to phytoplankton, present in the water. There are multiple retrieval algorithms for estimating Chl-a and aggregating the data over time. This data set is a reprocessed copy of 9km monthly and 8-day versions produced globally by NASA, adjusted for the Southern Ocean south of latitude 30S. The full methodology is described in Johnson, R., Strutton, P.G., Wright, S.W., McMinn, A., Meiners, K.M., 2013. Three improved satellite chlorophyll algorithms for the Southern Ocean. Journal of Geophysical Research: Oceans. doi: 10.1002/jgrc.20270. It is expected that the data set will be periodically updated with contemporary data as it becomes available. There are four sub-streams within this data set. A monthly and an 8-day series for MODIS/Aqua and similarly for SeaWIFS. Note that SeaWIFS ceased operation in late 2010 so there will be no further SeaWIFS data. The data represented by this record is weekly data for SeaWIFS.

The Aqua and Orbview satellites carry a MODIS and SeaWIFS sensors (respectively) that observes sunlight reflected from within the ocean surface layer at multiple wavelengths. These multi-spectral measurements are used to infer the concentration of chlorophyll-a (Chl-a), most typically due to phytoplankton, present in the water. There are multiple retrieval algorithms for estimating Chl-a and aggregating the data over time. This data set is a reprocessed copy of 9km monthly and 8-day versions produced globally by NASA, adjusted for the Southern Ocean south of latitude 30S. The full methodology is described in Johnson, R., Strutton, P.G., Wright, S.W., McMinn, A., Meiners, K.M., 2013. Three improved satellite chlorophyll algorithms for the Southern Ocean. Journal of Geophysical Research: Oceans. doi: 10.1002/jgrc.20270. It is expected that the data set will be periodically updated with contemporary data as it becomes available. There are four sub-streams within this data set. A monthly and an 8-day series for MODIS/Aqua and similarly for SeaWIFS. Note that SeaWIFS ceased operation in late 2010 so there will be no further SeaWIFS data. The data represented by this record is weekly data for MODIS/Aqua.

The National Reef Monitoring Network brings together shallow reef surveys conducted around Australia into a centralised database. The IMOS National Reef Monitoring Network sub-Facility collates, cleans, stores and makes this data rapidly available from contributors including: Reef Life Survey, Parks Australia, Department of Biodiversity, Conservation and Attractions (Western Australia), Department of Environment, Water and Natural Resources (South Australia), Department of Primary Industries (New South Wales), Tasmanian Parks and Wildlife Service and Parks Victoria. The data provided by the National Reef Monitoring Network contributes to establishing and supporting national marine baselines, and assisting with the management of Commonwealth and State marine reserves. Reef Life Survey (RLS) and the Australian Temperate Reef Network (ATRC) aims to improve biodiversity conservation and the sustainable management of marine resources by coordinating surveys of rocky and coral reefs using scientific methods, with the ultimate goal to improve coastal stewardship. Our activities depend on the skills of marine scientists, experienced and motivated recreational SCUBA divers, partnerships with management agencies and university researchers, and active input from the ATRC partners and RLS Advisory Committee. RLS and ATRC data are freely available to the public for non-profit purposes, so not only managers, but also groups such as local dive clubs or schools may use these data to look at changes over time in their own local reefs. By making data freely available and through public outputs, RLS and ATRC aims to raise broader community awareness of the status of Australia's marine biodiversity and associated conservation issues. This dataset contains records of mobile macroinvertebrates collected by Reef Life Survey (RLS) and Australian Temperate Reef Collaboration (ATRC) divers and partners along 50m transects on shallow rocky and coral reefs using standard methods. Abundance information is available for all species recorded within quantitative survey limits (50 x 1 m swathes either side of the transect line, each distinguished as a 'Block'), with divers searching the reef surface (including cracks) carefully for hidden invertebrates such as sea stars, urchins, gastropods, lobsters, crabs etc. These observations are recorded concurrently with the cryptobenthic fish observations and together make up the 'Method 2' component of the surveys. For this method, typically one 'Block' is completed per 50 m transect for the program ATRC and 2 blocks are completed for RLS - although exceptions to this rule exist. This dataset supersedes the RLS specific "Reef Life Survey (RLS): Invertebrates" collection that was available at https://catalogue-rls.imas.utas.edu.au/geonetwork/srv/en/metadata.show?uuid=60978150-1641-11dd-a326-00188b4c0af8 (provision of data was stopped in June 2021).

The Aqua and Orbview satellites carry a MODIS and SeaWIFS sensors (respectively) that observes sunlight reflected from within the ocean surface layer at multiple wavelengths. These multi-spectral measurements are used to infer the concentration of chlorophyll-a (Chl-a), most typically due to phytoplankton, present in the water. There are multiple retrieval algorithms for estimating Chl-a and aggregating the data over time. This data set is a reprocessed copy of 9km monthly and 8-day versions produced globally by NASA, adjusted for the Southern Ocean south of latitude 30S. The full methodology is described in Johnson, R., Strutton, P.G., Wright, S.W., McMinn, A., Meiners, K.M., 2013. Three improved satellite chlorophyll algorithms for the Southern Ocean. Journal of Geophysical Research: Oceans. doi: 10.1002/jgrc.20270. It is expected that the data set will be periodically updated with contemporary data as it becomes available. There are four sub-streams within this data set. A monthly and an 8-day series for MODIS/Aqua and similarly for SeaWIFS. Note that SeaWIFS ceased operation in late 2010 so there will be no further SeaWIFS data. The data represented by this record is monthly data for SeaWIFS.

The National Reef Monitoring Network brings together shallow reef surveys conducted around Australia into a centralised database. The IMOS National Reef Monitoring Network sub-Facility collates, cleans, stores and makes this data rapidly available from contributors including: Reef Life Survey, Parks Australia, Department of Biodiversity, Conservation and Attractions (Western Australia), Department of Environment, Water and Natural Resources (South Australia), Department of Primary Industries (New South Wales), Tasmanian Parks and Wildlife Service and Parks Victoria. The data provided by the National Reef Monitoring Network contributes to establishing and supporting national marine baselines, and assisting with the management of Commonwealth and State marine reserves. Reef Life Survey (RLS) and the Australian Temperate Reef Network (ATRC) aims to improve biodiversity conservation and the sustainable management of marine resources by coordinating surveys of rocky and coral reefs using scientific methods, with the ultimate goal to improve coastal stewardship. Our activities depend on the skills of marine scientists, experienced and motivated recreational SCUBA divers, partnerships with management agencies and university researchers, and active input from the ATRC partners and RLS Advisory Committee. RLS and ATRC data are freely available to the public for non-profit purposes, so not only managers, but also groups such as local dive clubs or schools may use these data to look at changes over time in their own local reefs. By making data freely available and through public outputs, RLS and ATRC aims to raise broader community awareness of the status of Australia's marine biodiversity and associated conservation issues. This dataset shows the locations of Reef Life Survey (RLS) and Australian Reef Collaboration Network (ATRC) sites on shallow rocky and coral reefs, worldwide. Multiple transects (surveys) are usually surveyed within sites on any given date. Sites traditionally need a minimum of 200 m separation to be classified as a different site, unless separated by a significant geological feature or reef habitat discontinuity.

The Aqua and Orbview satellites carry a MODIS and SeaWIFS sensors (respectively) that observes sunlight reflected from within the ocean surface layer at multiple wavelengths. These multi-spectral measurements are used to infer the concentration of chlorophyll-a (Chl-a), most typically due to phytoplankton, present in the water. There are multiple retrieval algorithms for estimating Chl-a and aggregating the data over time. This data set is a reprocessed copy of 9km monthly and 8-day versions produced globally by NASA, adjusted for the Southern Ocean south of latitude 30S. The full methodology is described in Johnson, R., Strutton, P.G., Wright, S.W., McMinn, A., Meiners, K.M., 2013. Three improved satellite chlorophyll algorithms for the Southern Ocean. Journal of Geophysical Research: Oceans. doi: 10.1002/jgrc.20270. It is expected that the data set will be periodically updated with contemporary data as it becomes available. There are four sub-streams within this data set. A monthly and an 8-day series for MODIS/Aqua and similarly for SeaWIFS. Note that SeaWIFS ceased operation in late 2010 so there will be no further SeaWIFS data. The data represented by this record is monthly data for MODIS/Aqua.

The National Reef Monitoring Network brings together shallow reef surveys conducted around Australia into a centralised database. The IMOS National Reef Monitoring Network sub-Facility collates, cleans, stores and makes this data rapidly available from contributors including: Reef Life Survey, Parks Australia, Department of Biodiversity, Conservation and Attractions (Western Australia), Department of Environment, Water and Natural Resources (South Australia), Department of Primary Industries (New South Wales), Tasmanian Parks and Wildlife Service and Parks Victoria. The data provided by the National Reef Monitoring Network contributes to establishing and supporting national marine baselines, and assisting with the management of Commonwealth and State marine reserves. Reef Life Survey (RLS) and the Australian Temperate Reef Network (ATRC) aims to improve biodiversity conservation and the sustainable management of marine resources by coordinating surveys of rocky and coral reefs using scientific methods, with the ultimate goal to improve coastal stewardship. Our activities depend on the skills of marine scientists, experienced and motivated recreational SCUBA divers, partnerships with management agencies and university researchers, and active input from the ATRC partners and RLS Advisory Committee. RLS and ATRC data are freely available to the public for non-profit purposes, so not only managers, but also groups such as local dive clubs or schools may use these data to look at changes over time in their own local reefs. By making data freely available and through public outputs, RLS and ATRC aims to raise broader community awareness of the status of Australia's marine biodiversity and associated conservation issues. This dataset contains records of bony fishes and elasmobranchs collected by Reef Life Survey (RLS) and Australian Temperate Reef Collaboration (ATRC) divers and partners along 50m transects on shallow rocky and coral reefs using standard methods. Abundance information is available for all species recorded within quantitative survey limits (50 x 5 m swathes either side of the transect line, each distinguished as a 'Block'), with size and biomass data also included when available. These observations form the Method 1 component of the surveys. This dataset supersedes the RLS specific "Reef Life Survey (RLS): Global reef fish dataset" collection that was available at https://catalogue-rls.imas.utas.edu.au/geonetwork/srv/en/metadata.show?uuid=9c766140-9e72-4bfb-8f04-d51038355c59 (provision of data was stopped in June 2021).